New components of the Drosophila fusome suggest it plays novel roles in signaling and transport

Daniel V. Lighthouse, Michael Buszczak, Allan C. Spradling

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

The fusome plays an essential role in prefollicular germ cell development within insects such as Drosophila melanogaster. Alpha-spectrin and the adducin-like protein Hu-li tai shao (Hts) are required to maintain fusome integrity, synchronize asymmetric cystocyte mitoses, form interconnected 16-cell germline cysts, and specify the initial cell as the oocyte. By screening a library of protein trap lines, we identified 14 new fusome-enriched proteins, including many associated with its characteristic vesicles. Our studies reveal that fusomes change during development and contain recycling endosomal and lysosomal compartments in females but not males. A significant number of fusome components are dispensable, because genetic disruption of tropomodulin, ferritin-1 heavy chain, or scribble, does not alter fusome structure or female fertility. In contrast, rab11 is required to maintain the germline stem cells, and to maintain the vesicle content of the spectrosome, suggesting that the fusome mediates intercellular signals that depend on the recycling endosome.

Original languageEnglish (US)
Pages (from-to)59-71
Number of pages13
JournalDevelopmental Biology
Volume317
Issue number1
DOIs
StatePublished - May 1 2008

Keywords

  • Drosophila
  • Fusome
  • Rab11
  • Stem cell

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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