New fluorescent tools for watching nanometer-scale conformational changes of single molecules

Erdal Toprak, Paul R. Selvin

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Single-molecule biophysics has been serving biology for more than two decades. Fluorescence microscopy is one of the most commonly used tools to identify molecules of interest and to visualize biological events. Here we describe some of the most commonly used fluorescence imaging tools to measure nanoscale movements and the rotational dynamics of biomolecules.

Original languageEnglish (US)
Pages (from-to)349-369
Number of pages21
JournalAnnual Review of Biophysics and Biomolecular Structure
Volume36
DOIs
StatePublished - 2007

Fingerprint

Biophysics
Optical Imaging
Fluorescence Microscopy
Molecules
Fluorescence microscopy
Biomolecules
Fluorescence
Imaging techniques

Keywords

  • Fluorescence microscopy
  • Measuring step sizes
  • Motility mechanism
  • Motor proteins

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology

Cite this

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