Abstract
The 19F NMR is gaining interest as a tool for diverse physiological and pharmaceutical investigations. The relatively high sensitivity of 19F and lack of interfering background signal in the body have enabled the observation of exogenously administered agents and their metabolites. The high gyromagnetic ratio often allows the use of existing proton NMR instrumentation with the minimum of component adjustments. 19F NMR is particularly attractive for in vivo applications since there is essentially no endogenous signal from tissues. 19F is exceptionally sensitive to molecular and microenvironmental changes as exemplified by the many 19F-based reporter molecules designed to interrogate physiological phenomena in vivo. The presence of the 19F atom may modulate molecular properties, most notably hydrophobicity and this becomes more significant for multiple fluorines, as encountered in CF3 groups.
Original language | English (US) |
---|---|
Pages (from-to) | 25-49 |
Number of pages | 25 |
Journal | Progress in Nuclear Magnetic Resonance Spectroscopy |
Volume | 70 |
DOIs | |
State | Published - Apr 2013 |
Keywords
- Enzyme activity
- Fluorine
- Hypoxia
- Metal ions
- NMR
- pH
- β-Galactosidase
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Nuclear and High Energy Physics
- Spectroscopy