New immunoglobulin IgG allotypes and haplotypes found in wild mice with monoclonal anti-allotope antibodies

C. M. Huang, M. Parsons, E. K. Wakeland, K. Moriwaki, L. A. Herzenberg

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Sera from 156 wild mice (Mus musculus L.) collected from parts of Eurasia, Nothern Africa, and the Americas were tested in solid-phase radioimmunoassays for reactivity with 20 monoclonal anti-allotope antibodies directed against gene products of the Igh-1, Igh-3, and Igh-4 loci. Most of the wild mice have Igh phenotypes similar to those of inbred strains or heterozygotes thereof, but frequently the wild mice showed unique combinations of allotypic determinants (allotypes) for a given immunoglobulin allele and unique combinations of allotypes on a given chromosome (haplotypes) not seen in inbred strains. We found new allotypes of the Igh-1 and Igh-4 loci. Mice from three regions (Poland, Taiwan, and Japan) possess combinations of allotypes indicating that recombination, gene conversion, or other gene duplication events have occurred in portions of the Igh constant region gene complex. Most interestingly, sera of three mice from Egypt possess immunoglobulin molecules appearing to result from an intragenic recombination event involving either Igh-1d or Igh-3d with Igh-1b. Two mice from Pakistan and one from Seychelles Island in the Indian Ocean also showed similar unusual phenotypes. These mice possess two CH2 IgH-1b and two CH2 Igh-1a/CH2 Igh-1d determinants, suggesting that these variant immunoglobulin arose from recombinations within the CH2 domain.

Original languageEnglish (US)
Pages (from-to)661-667
Number of pages7
JournalJournal of Immunology
Volume128
Issue number2
StatePublished - 1982

Fingerprint

Haplotypes
Immunoglobulins
Anti-Idiotypic Antibodies
Immunoglobulin G
Monoclonal Antibodies
Genetic Recombination
Seychelles
Indian Ocean Islands
Phenotype
Gene Conversion
Gene Duplication
Egypt
Pakistan
Poland
Heterozygote
Serum
Taiwan
Genes
Radioimmunoassay
Japan

ASJC Scopus subject areas

  • Immunology

Cite this

New immunoglobulin IgG allotypes and haplotypes found in wild mice with monoclonal anti-allotope antibodies. / Huang, C. M.; Parsons, M.; Wakeland, E. K.; Moriwaki, K.; Herzenberg, L. A.

In: Journal of Immunology, Vol. 128, No. 2, 1982, p. 661-667.

Research output: Contribution to journalArticle

Huang, C. M. ; Parsons, M. ; Wakeland, E. K. ; Moriwaki, K. ; Herzenberg, L. A. / New immunoglobulin IgG allotypes and haplotypes found in wild mice with monoclonal anti-allotope antibodies. In: Journal of Immunology. 1982 ; Vol. 128, No. 2. pp. 661-667.
@article{7fea069199e444698e1b631a75e78a7e,
title = "New immunoglobulin IgG allotypes and haplotypes found in wild mice with monoclonal anti-allotope antibodies",
abstract = "Sera from 156 wild mice (Mus musculus L.) collected from parts of Eurasia, Nothern Africa, and the Americas were tested in solid-phase radioimmunoassays for reactivity with 20 monoclonal anti-allotope antibodies directed against gene products of the Igh-1, Igh-3, and Igh-4 loci. Most of the wild mice have Igh phenotypes similar to those of inbred strains or heterozygotes thereof, but frequently the wild mice showed unique combinations of allotypic determinants (allotypes) for a given immunoglobulin allele and unique combinations of allotypes on a given chromosome (haplotypes) not seen in inbred strains. We found new allotypes of the Igh-1 and Igh-4 loci. Mice from three regions (Poland, Taiwan, and Japan) possess combinations of allotypes indicating that recombination, gene conversion, or other gene duplication events have occurred in portions of the Igh constant region gene complex. Most interestingly, sera of three mice from Egypt possess immunoglobulin molecules appearing to result from an intragenic recombination event involving either Igh-1d or Igh-3d with Igh-1b. Two mice from Pakistan and one from Seychelles Island in the Indian Ocean also showed similar unusual phenotypes. These mice possess two CH2 IgH-1b and two CH2 Igh-1a/CH2 Igh-1d determinants, suggesting that these variant immunoglobulin arose from recombinations within the CH2 domain.",
author = "Huang, {C. M.} and M. Parsons and Wakeland, {E. K.} and K. Moriwaki and Herzenberg, {L. A.}",
year = "1982",
language = "English (US)",
volume = "128",
pages = "661--667",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "2",

}

TY - JOUR

T1 - New immunoglobulin IgG allotypes and haplotypes found in wild mice with monoclonal anti-allotope antibodies

AU - Huang, C. M.

AU - Parsons, M.

AU - Wakeland, E. K.

AU - Moriwaki, K.

AU - Herzenberg, L. A.

PY - 1982

Y1 - 1982

N2 - Sera from 156 wild mice (Mus musculus L.) collected from parts of Eurasia, Nothern Africa, and the Americas were tested in solid-phase radioimmunoassays for reactivity with 20 monoclonal anti-allotope antibodies directed against gene products of the Igh-1, Igh-3, and Igh-4 loci. Most of the wild mice have Igh phenotypes similar to those of inbred strains or heterozygotes thereof, but frequently the wild mice showed unique combinations of allotypic determinants (allotypes) for a given immunoglobulin allele and unique combinations of allotypes on a given chromosome (haplotypes) not seen in inbred strains. We found new allotypes of the Igh-1 and Igh-4 loci. Mice from three regions (Poland, Taiwan, and Japan) possess combinations of allotypes indicating that recombination, gene conversion, or other gene duplication events have occurred in portions of the Igh constant region gene complex. Most interestingly, sera of three mice from Egypt possess immunoglobulin molecules appearing to result from an intragenic recombination event involving either Igh-1d or Igh-3d with Igh-1b. Two mice from Pakistan and one from Seychelles Island in the Indian Ocean also showed similar unusual phenotypes. These mice possess two CH2 IgH-1b and two CH2 Igh-1a/CH2 Igh-1d determinants, suggesting that these variant immunoglobulin arose from recombinations within the CH2 domain.

AB - Sera from 156 wild mice (Mus musculus L.) collected from parts of Eurasia, Nothern Africa, and the Americas were tested in solid-phase radioimmunoassays for reactivity with 20 monoclonal anti-allotope antibodies directed against gene products of the Igh-1, Igh-3, and Igh-4 loci. Most of the wild mice have Igh phenotypes similar to those of inbred strains or heterozygotes thereof, but frequently the wild mice showed unique combinations of allotypic determinants (allotypes) for a given immunoglobulin allele and unique combinations of allotypes on a given chromosome (haplotypes) not seen in inbred strains. We found new allotypes of the Igh-1 and Igh-4 loci. Mice from three regions (Poland, Taiwan, and Japan) possess combinations of allotypes indicating that recombination, gene conversion, or other gene duplication events have occurred in portions of the Igh constant region gene complex. Most interestingly, sera of three mice from Egypt possess immunoglobulin molecules appearing to result from an intragenic recombination event involving either Igh-1d or Igh-3d with Igh-1b. Two mice from Pakistan and one from Seychelles Island in the Indian Ocean also showed similar unusual phenotypes. These mice possess two CH2 IgH-1b and two CH2 Igh-1a/CH2 Igh-1d determinants, suggesting that these variant immunoglobulin arose from recombinations within the CH2 domain.

UR - http://www.scopus.com/inward/record.url?scp=0020046430&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020046430&partnerID=8YFLogxK

M3 - Article

VL - 128

SP - 661

EP - 667

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 2

ER -