New insights into the mitochondrial carnitine palmitoyltransferase enzyme system

J. D. McGarry, A. Sen, V. Esser, K. F. Woeltje, B. Weis, D. W. Foster

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Dissection of the mitochondrial carnitine palmitoyltransferase (CPT) enzyme system in terms of its structure/function relationships has proved to be a formidable task. Although no one formulation has gained universal agreement we believe that the weight of evidence supports a model with the following features: a) in any given tissue CPT I and CPT II are distinct proteins: b) CPT I, unlike CPT II, is detergent labile: c) within a species CPT II is expressed body wide, whereas CPT I exists as tissue specific isoforms; d) malonyl-CoA and other CPT I inhibitors probably interact at the catalytic center of the enzyme, not with a regulatory subunit. The amino acid sequences of rat and human CPT II (deduced from cDNA clones) show them to be similar proteins (> 80% identity) but encoded by mRNAs of significantly different sizes. Efforts to clone and sequence the cDNA for rat liver CPT I are presently underway.

Original languageEnglish (US)
Pages (from-to)77-84
Number of pages8
JournalBiochimie
Volume73
Issue number1
DOIs
StatePublished - 1991

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Carnitine O-Palmitoyltransferase
Enzymes
Rats
Complementary DNA
Clone Cells
Tissue
Malonyl Coenzyme A
Dissection
Detergents
Liver
Amino Acid Sequence
Protein Isoforms
Proteins

Keywords

  • carnitine palmitoyltransferase
  • isoforms
  • structure/function

ASJC Scopus subject areas

  • Biochemistry

Cite this

New insights into the mitochondrial carnitine palmitoyltransferase enzyme system. / McGarry, J. D.; Sen, A.; Esser, V.; Woeltje, K. F.; Weis, B.; Foster, D. W.

In: Biochimie, Vol. 73, No. 1, 1991, p. 77-84.

Research output: Contribution to journalArticle

McGarry, J. D. ; Sen, A. ; Esser, V. ; Woeltje, K. F. ; Weis, B. ; Foster, D. W. / New insights into the mitochondrial carnitine palmitoyltransferase enzyme system. In: Biochimie. 1991 ; Vol. 73, No. 1. pp. 77-84.
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