TY - JOUR
T1 - NF-Κ and ERK-signaling pathways contribute to the gene expression induced by cag PAI-postive-Helicobacter pylori infection
AU - Shibata, Wataru
AU - Hirata, Yoshihiro
AU - Yoshida, Haruhiko
AU - Otsuka, Motoyuki
AU - Hoshida, Yujin
AU - Ogura, Keiji
AU - Maeda, Shin
AU - Ohmae, Tomoya
AU - Yanai, Ayako
AU - Mitsuno, Yuzo
AU - Seki, Naohiko
AU - Kawabe, Takao
AU - Omata, Masao
PY - 2005/10/21
Y1 - 2005/10/21
N2 - Aim: To elucidate the sequential gene expression profile in AGS cells co-cultured with wild-type Helicobacter pylori (H pylori) as a model of H pylori-infected gastric epithelium, and to further examine the contribution of cag-pathogenicity islands (cagPAI)-coding type IV secretion system and the two pathways, nuclear factor kappa B (NF-κB) and extracellular signal-regulated kinases (ERK) on wild-type H pylori-induced gene expression. Methods: Gene expression profiles induced by H pylori were evaluated in AGS gastric epithelial cells using cDNA microarray, which were present in the 4 600 independent clones picked up from the human gastric tissue. We also analyzed the contribution of NF-κB and ERK signaling on H pylori-induced gene expression by using inhibitors of specific signal pathways. The isogenic mutant with disrupted cagE (Δ cagE) was used to elucidate the role of cagPAI-encoding type IV secretion system in the gene expression profile. Results: According to the expression profile, the genes were classified into four clusters. Among them, the clusters characterized by continuous upregulation were most conspicuous, and it contained many signal transducer activity-associated genes. The role of cagPAI on cultured cells was also investigated using isogenic mutant cagE, which carries non-functional cagPAI. Then the upregulation of more than 80% of the induced genes (476/566) was found to depend on cagPAI. Signal transducer pathway through NF-κB or ERK are the major pathways which are known to be activated by cagPAI-positive H pylori. The role of these pathways in the whole signal activation by cagPAI-positive H pylori was analyzed. The specific inhibitors against NF-κB or ERK pathway blocked the activation of gene expression in 65% (367/566) or 76% (429/566) of the genes whose activation appealed to depend on cagPAI. Conclusion: These results suggest that more than half of the genes induced by cagPAI-positive H pylori depend on NF-κB and ERK signaling activation, and these pathways may play a role in the gene expression induced by host-bacterial interaction which may associate with H pylori-related gastro-duodenal diseases.
AB - Aim: To elucidate the sequential gene expression profile in AGS cells co-cultured with wild-type Helicobacter pylori (H pylori) as a model of H pylori-infected gastric epithelium, and to further examine the contribution of cag-pathogenicity islands (cagPAI)-coding type IV secretion system and the two pathways, nuclear factor kappa B (NF-κB) and extracellular signal-regulated kinases (ERK) on wild-type H pylori-induced gene expression. Methods: Gene expression profiles induced by H pylori were evaluated in AGS gastric epithelial cells using cDNA microarray, which were present in the 4 600 independent clones picked up from the human gastric tissue. We also analyzed the contribution of NF-κB and ERK signaling on H pylori-induced gene expression by using inhibitors of specific signal pathways. The isogenic mutant with disrupted cagE (Δ cagE) was used to elucidate the role of cagPAI-encoding type IV secretion system in the gene expression profile. Results: According to the expression profile, the genes were classified into four clusters. Among them, the clusters characterized by continuous upregulation were most conspicuous, and it contained many signal transducer activity-associated genes. The role of cagPAI on cultured cells was also investigated using isogenic mutant cagE, which carries non-functional cagPAI. Then the upregulation of more than 80% of the induced genes (476/566) was found to depend on cagPAI. Signal transducer pathway through NF-κB or ERK are the major pathways which are known to be activated by cagPAI-positive H pylori. The role of these pathways in the whole signal activation by cagPAI-positive H pylori was analyzed. The specific inhibitors against NF-κB or ERK pathway blocked the activation of gene expression in 65% (367/566) or 76% (429/566) of the genes whose activation appealed to depend on cagPAI. Conclusion: These results suggest that more than half of the genes induced by cagPAI-positive H pylori depend on NF-κB and ERK signaling activation, and these pathways may play a role in the gene expression induced by host-bacterial interaction which may associate with H pylori-related gastro-duodenal diseases.
KW - Cag-pathogenicity islands
KW - Cluster analysis
KW - Helicobacter pylori
KW - Signal transduction
KW - cDNA microarray
UR - http://www.scopus.com/inward/record.url?scp=30644474993&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=30644474993&partnerID=8YFLogxK
U2 - 10.3748/wjg.v11.i39.6134
DO - 10.3748/wjg.v11.i39.6134
M3 - Article
C2 - 16273640
AN - SCOPUS:30644474993
SN - 1007-9327
VL - 11
SP - 6134
EP - 6143
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
IS - 39
ER -