NF-κB2 is required for the establishment of central tolerance through an Aire-dependent pathway

Mingzhao Zhu, Robert K. Chin, Peter A. Christiansen, James C. Lo, Xiaojuan Liu, Carl Ware, Ulrich Siebenlist, Yang Xin Fu

Research output: Contribution to journalArticlepeer-review

98 Scopus citations

Abstract

NF-κB2-deficient mice have impaired T and B cell responses. We found, however, that in these mice there was severe infiltration of lymphocytes into multiple organs and increased activity of autoantibodies to peripheral tissue antigens in a manner similar to that of autoimmune regulator-deficient (Aire-deficient) mice. We further demonstrated that NF-κB2 was required for thymic Aire gene transcriptional regulation. The Nfkb2-/- thymus had distinct cortical and medullar structures, but reduced Aire and target gene expression of peripheral tissue antigens. Engraftment of Nfkb2-/- thymic stroma to nude mice recapitulated the autoimmune phenotype of the native Nfkb2-/- mice, confirming a key defect in central tolerance. Lymphotoxin β receptor (LTβR) ligation-induced Aire gene expression was also largely abolished in the absence of NF-κB2. Thus NF-κB2 downstream of LTβR plays an important role in the regulation of central tolerance in an Aire-dependent manner.

Original languageEnglish (US)
Pages (from-to)2964-2971
Number of pages8
JournalJournal of Clinical Investigation
Volume116
Issue number11
DOIs
StatePublished - Nov 1 2006

ASJC Scopus subject areas

  • Medicine(all)

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