Nf1 has an essential role in endothelial cells

Aaron D. Gitler, Yuan Zhu, Fraz A. Ismat, Min Min Lu, Yasutaka Yamauchi, Luis F. Parada, Jonathan A. Epstein

Research output: Contribution to journalArticle

139 Scopus citations

Abstract

Neurofibromatosis type 1 (NF1) or von Recklinghausen neurofibromatosis is a genetic disorder that occurs in 1 of 4000 births and is characterized by benign and malignant tumors. Cardiovascular defects also contribute to NF1, though the pathogenesis is still unclear. Deficiency in neurofibromin (encoded by Nf1) in mice results in mid-embryonic lethality owing to cardiac abnormalities previously thought to be secondary to cardiac neural-crest defects. Using tissue-specific gene inactivation, we show that endothelial-specific inactivation of Nf1 recapitulates key aspects of the complete null phenotype, including multiple cardiovascular abnormalities involving the endocardial cushions and myocardium. This phenotype is associated with an elevated level of ras signaling in Nf1-/- endothelial cells and greater nuclear localization of the transcription factor Nfatc1. Inactivation of Nf1 in the neural crest does not cause cardiac defects but results in tumors of neural-crest origin resembling those seen in humans with NF1. These results establish a new and essential role for Nf1 in endothelial cells and confirm the requirement for neurofibromin in the neural crest.

Original languageEnglish (US)
Pages (from-to)75-79
Number of pages5
JournalNature genetics
Volume33
Issue number1
DOIs
StatePublished - Jan 1 2003

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Nf1 has an essential role in endothelial cells'. Together they form a unique fingerprint.

  • Cite this

    Gitler, A. D., Zhu, Y., Ismat, F. A., Lu, M. M., Yamauchi, Y., Parada, L. F., & Epstein, J. A. (2003). Nf1 has an essential role in endothelial cells. Nature genetics, 33(1), 75-79. https://doi.org/10.1038/ng1059