Nf1+/- mast cells induce neurofibroma like phenotypes through secreted TGF-β signaling

Feng Chun Yang, Shi Chen, Travis Clegg, Xiaohong Li, Trent Morgan, Selina A. Estwick, Jin Yuan, Waleed Khalaf, Sarah Burgin, Jeff Travers, Luis F. Parada, David A. Ingram, D. Wade Clapp

Research output: Contribution to journalArticle

95 Scopus citations

Abstract

Neurofibromas are common tumors found in neurofibromatosis type 1 (NF1) patients. These complex tumors are composed of Schwann cells, mast cells, fibroblasts and perineurial cells embedded in collagen that provide a lattice for tumor invasion. Genetic studies demonstrate that in neurofibromas, nullizygous loss of Nf1 in Schwann cells and haploinsufficiency of Nf1 in non-neuronal cells are required for tumorigenesis. Fibroblasts are a major cellular constituent in neurofibromas and are a source of collagen that constitutes ∼50% of the dry weight of the tumor. Here, we show that two of the prevalent heterozygous cells found in neurofibromas, mast cells and fibroblasts interact directly to contribute to tumor phenotype. Nf1+/- mast cells secrete elevated concentrations of the profibrotic transforming growth factor-beta (TGF-β). In response to TGF-β, both murine Nf1+/- fibroblasts and fibroblasts from human neurofibromas proliferate and synthesize excessive collagen, a hallmark of neurofibromas. We also establish that the TGF-β response occurs via hyperactivation of a novel Ras-c-abl signaling pathway. Genetic or pharmacological inhibition of c-abl reverses fibroblast proliferation and collagen synthesis to wild-type levels.These studies identify a novel molecular target to inhibit neurofibroma formation.

Original languageEnglish (US)
Pages (from-to)2421-2437
Number of pages17
JournalHuman molecular genetics
Volume15
Issue number16
DOIs
StatePublished - Aug 15 2006

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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    Yang, F. C., Chen, S., Clegg, T., Li, X., Morgan, T., Estwick, S. A., Yuan, J., Khalaf, W., Burgin, S., Travers, J., Parada, L. F., Ingram, D. A., & Clapp, D. W. (2006). Nf1+/- mast cells induce neurofibroma like phenotypes through secreted TGF-β signaling. Human molecular genetics, 15(16), 2421-2437. https://doi.org/10.1093/hmg/ddl165