NHE3: A Na+/H+ exchanger isoform of renal brush border

Daniel Biemesderfer, John Pizzonia, Ali Abu-Alfa, Markus Exner, Robert Reilly, Peter Igarashi, Peter S. Aronson

Research output: Contribution to journalArticle

361 Citations (Scopus)

Abstract

Na+/H+ exchangers in the brush-border (luminal, apical) membrane of renal proximal tubules are responsible for active, transcellular reabsorption of NaHCO3 and NaCl. Although well characterized kinetically, the protein that mediates Na+/H+ exchange in the renal brush border has not been identified. Several Na+/H+ exchanger genes, including NHE1, NHE2, NHE3, and NHE4, are expressed in the kidney. To identify the NHE3 gene product and to determine its cellular and subcellular localization in the rabbit kidney, an NHE3-isoform-specific antibody was prepared. Guinea pigs were immunized with purified fusion protein containing the carboxy-terminal 40 amino acids of NHE3 (fpNHE3-C40). After affinity purification, immune sera demonstrated specific reactivity to the NHE3 sequence within the fusion protein as well as to an 80-kDa polypeptide expressed in NHE3-transfected LAP1 cells. Western blot analysis showed that anti-fpNHE3-C40 specifically reacted with an 80-kDa protein that is relatively enriched in renal brush-border membrane compared with basolateral membrane. Immunocytochemical studies confirmed that the Na+/H+ exchanger isoform NHE3 is expressed along the microvillar membrane of the brush border of proximal tubule cells in the rabbit kidney.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume265
Issue number5 34-5
StatePublished - Nov 1993

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Sodium-Hydrogen Antiporter
Microvilli
Protein Isoforms
Kidney
Membranes
Proteins
Rabbits
Proximal Kidney Tubule
Genes
Immune Sera
Guinea Pigs
Western Blotting
Amino Acids
Peptides
Antibodies

Keywords

  • Acidification
  • Intracellular pH
  • Membrane protein

ASJC Scopus subject areas

  • Physiology

Cite this

Biemesderfer, D., Pizzonia, J., Abu-Alfa, A., Exner, M., Reilly, R., Igarashi, P., & Aronson, P. S. (1993). NHE3: A Na+/H+ exchanger isoform of renal brush border. American Journal of Physiology - Renal Fluid and Electrolyte Physiology, 265(5 34-5).

NHE3 : A Na+/H+ exchanger isoform of renal brush border. / Biemesderfer, Daniel; Pizzonia, John; Abu-Alfa, Ali; Exner, Markus; Reilly, Robert; Igarashi, Peter; Aronson, Peter S.

In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology, Vol. 265, No. 5 34-5, 11.1993.

Research output: Contribution to journalArticle

Biemesderfer, D, Pizzonia, J, Abu-Alfa, A, Exner, M, Reilly, R, Igarashi, P & Aronson, PS 1993, 'NHE3: A Na+/H+ exchanger isoform of renal brush border', American Journal of Physiology - Renal Fluid and Electrolyte Physiology, vol. 265, no. 5 34-5.
Biemesderfer D, Pizzonia J, Abu-Alfa A, Exner M, Reilly R, Igarashi P et al. NHE3: A Na+/H+ exchanger isoform of renal brush border. American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 1993 Nov;265(5 34-5).
Biemesderfer, Daniel ; Pizzonia, John ; Abu-Alfa, Ali ; Exner, Markus ; Reilly, Robert ; Igarashi, Peter ; Aronson, Peter S. / NHE3 : A Na+/H+ exchanger isoform of renal brush border. In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 1993 ; Vol. 265, No. 5 34-5.
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