Nigericin-induced charge transfer across membranes

V. S. Markin, V. S. Sokolov, L. I. Boguslavsky, L. S. Jaguzhinsky

Research output: Contribution to journalArticle

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Abstract

The electric properties of the bilayer lecithin membranes have been studied in the presence of the antibiotic nigericin. When the antibiotic concentration is about 10-6m the conductivity of the BLM is increased up to 10-7 ohm-1 cm-2. The potassium ion concentration gradient gives rise to a transmembrane potential of the order of 40 mV per 10-fold concentration gradient with the side of the higher potassium concentration negative. The transmembrane potential produced by the hydrogen ion concentration gradient is a function of the potassium ion concentration which is equal on both sides of the membrane. For low potassium ion concentrations the hydrogen potential has the expected polarity with the solution having higher concentration of protons negative. For potassium ion concentrations exceeding 0.03 m the hydrogen potential has the reverse polarity. This unexpected result cannot be accounted for in terms of the available simple hypotheses about the charge transport mechanism for nigericin in BLM. In order to account for the experimental results obtained, a theoretical approach has been developed based on the assumption that charge is transported across the membrane by nigericin dimers. The theoretical predictions are in satisfactory agreement with the experimental results. The model also yields some predictions which may be verified in future experiments.

Original languageEnglish (US)
Pages (from-to)23-45
Number of pages23
JournalThe Journal of Membrane Biology
Volume25
Issue number1
DOIs
StatePublished - Dec 1975

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Nigericin
Potassium
Membranes
Ions
Membrane Potentials
Anti-Bacterial Agents
Lecithins
Protons
Hydrogen

ASJC Scopus subject areas

  • Physiology
  • Cell Biology
  • Biophysics

Cite this

Markin, V. S., Sokolov, V. S., Boguslavsky, L. I., & Jaguzhinsky, L. S. (1975). Nigericin-induced charge transfer across membranes. The Journal of Membrane Biology, 25(1), 23-45. https://doi.org/10.1007/BF01868566

Nigericin-induced charge transfer across membranes. / Markin, V. S.; Sokolov, V. S.; Boguslavsky, L. I.; Jaguzhinsky, L. S.

In: The Journal of Membrane Biology, Vol. 25, No. 1, 12.1975, p. 23-45.

Research output: Contribution to journalArticle

Markin, VS, Sokolov, VS, Boguslavsky, LI & Jaguzhinsky, LS 1975, 'Nigericin-induced charge transfer across membranes', The Journal of Membrane Biology, vol. 25, no. 1, pp. 23-45. https://doi.org/10.1007/BF01868566
Markin VS, Sokolov VS, Boguslavsky LI, Jaguzhinsky LS. Nigericin-induced charge transfer across membranes. The Journal of Membrane Biology. 1975 Dec;25(1):23-45. https://doi.org/10.1007/BF01868566
Markin, V. S. ; Sokolov, V. S. ; Boguslavsky, L. I. ; Jaguzhinsky, L. S. / Nigericin-induced charge transfer across membranes. In: The Journal of Membrane Biology. 1975 ; Vol. 25, No. 1. pp. 23-45.
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