Nin 1p, a regulatory subunit of the 26S proteasome, is necessary for activation of Cdc28p kinase of Saccharomyces cerevisiae

Kin Ichiro Kominami, George N. DeMartino, Carolyn R. Moomaw, Clive A. Slaughter, Naoki Shimbara, Masahiro Fujimuro, Hideyoshi Yokosawa, Hiroshi Hisamatsu, Nobuyuki Tanahashi, Yoshihisa Shimizu, Keiji Tanaka, Akio Toh-e

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Abstract

The nin1-1 mutant of Saccharomyces cerevisiae cannot perform the G1/S and G2/M transitions at restrictive temperatures. At such temperatures, nin1-1 strains fail to activate histone H1 kinase after release from alpha factor-imposed G1 block and after release from hydroxyurea-imposed S block. The nin1-1 mutation shows synthetic lethality with certain cdc28 mutant alleles such as cdc28-1N. Two lines of evidence indicate that Nin1p is a component of the 26S proteasome complex: (i) Nin1p, as well as the known component of the 26S proteasome, shifted to the 26S proteasome peak in the glycerol density gradient after preincubation of crude extract with ATP-Mg 2+, and (ii) nin1-1 cells accumulated polyubiquitinated proteins under restrictive conditions. These results suggest that activation of Cdc28p kinase requires proteolysis. We have cloned a human cDNA encoding a regulatory subunit of the 26S proteasome, p31, which was found to be a homolog of Nin1p.

Original languageEnglish (US)
Pages (from-to)3105-3115
Number of pages11
JournalEMBO Journal
Volume14
Issue number13
StatePublished - 1995

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Yeast
Saccharomyces cerevisiae
Phosphotransferases
Chemical activation
Proteolysis
Temperature
Hydroxyurea
Complex Mixtures
Glycerol
Complementary DNA
Adenosine Triphosphate
Alleles
Mutation
ATP dependent 26S protease
Proteins

Keywords

  • 26S proteasome
  • Cdc28p kinase
  • Cell cycte
  • N1N1
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Kominami, K. I., DeMartino, G. N., Moomaw, C. R., Slaughter, C. A., Shimbara, N., Fujimuro, M., ... Toh-e, A. (1995). Nin 1p, a regulatory subunit of the 26S proteasome, is necessary for activation of Cdc28p kinase of Saccharomyces cerevisiae. EMBO Journal, 14(13), 3105-3115.

Nin 1p, a regulatory subunit of the 26S proteasome, is necessary for activation of Cdc28p kinase of Saccharomyces cerevisiae. / Kominami, Kin Ichiro; DeMartino, George N.; Moomaw, Carolyn R.; Slaughter, Clive A.; Shimbara, Naoki; Fujimuro, Masahiro; Yokosawa, Hideyoshi; Hisamatsu, Hiroshi; Tanahashi, Nobuyuki; Shimizu, Yoshihisa; Tanaka, Keiji; Toh-e, Akio.

In: EMBO Journal, Vol. 14, No. 13, 1995, p. 3105-3115.

Research output: Contribution to journalArticle

Kominami, KI, DeMartino, GN, Moomaw, CR, Slaughter, CA, Shimbara, N, Fujimuro, M, Yokosawa, H, Hisamatsu, H, Tanahashi, N, Shimizu, Y, Tanaka, K & Toh-e, A 1995, 'Nin 1p, a regulatory subunit of the 26S proteasome, is necessary for activation of Cdc28p kinase of Saccharomyces cerevisiae', EMBO Journal, vol. 14, no. 13, pp. 3105-3115.
Kominami, Kin Ichiro ; DeMartino, George N. ; Moomaw, Carolyn R. ; Slaughter, Clive A. ; Shimbara, Naoki ; Fujimuro, Masahiro ; Yokosawa, Hideyoshi ; Hisamatsu, Hiroshi ; Tanahashi, Nobuyuki ; Shimizu, Yoshihisa ; Tanaka, Keiji ; Toh-e, Akio. / Nin 1p, a regulatory subunit of the 26S proteasome, is necessary for activation of Cdc28p kinase of Saccharomyces cerevisiae. In: EMBO Journal. 1995 ; Vol. 14, No. 13. pp. 3105-3115.
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abstract = "The nin1-1 mutant of Saccharomyces cerevisiae cannot perform the G1/S and G2/M transitions at restrictive temperatures. At such temperatures, nin1-1 strains fail to activate histone H1 kinase after release from alpha factor-imposed G1 block and after release from hydroxyurea-imposed S block. The nin1-1 mutation shows synthetic lethality with certain cdc28 mutant alleles such as cdc28-1N. Two lines of evidence indicate that Nin1p is a component of the 26S proteasome complex: (i) Nin1p, as well as the known component of the 26S proteasome, shifted to the 26S proteasome peak in the glycerol density gradient after preincubation of crude extract with ATP-Mg 2+, and (ii) nin1-1 cells accumulated polyubiquitinated proteins under restrictive conditions. These results suggest that activation of Cdc28p kinase requires proteolysis. We have cloned a human cDNA encoding a regulatory subunit of the 26S proteasome, p31, which was found to be a homolog of Nin1p.",
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AU - Moomaw, Carolyn R.

AU - Slaughter, Clive A.

AU - Shimbara, Naoki

AU - Fujimuro, Masahiro

AU - Yokosawa, Hideyoshi

AU - Hisamatsu, Hiroshi

AU - Tanahashi, Nobuyuki

AU - Shimizu, Yoshihisa

AU - Tanaka, Keiji

AU - Toh-e, Akio

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