Nitric oxide and cutaneous active vasodilation during heat stress in humans

D. L. Kellogg, C. G. Crandall, Y. Liu, N. Charkoudian, J. M. Johnson

Research output: Contribution to journalArticle

228 Citations (Scopus)

Abstract

Whether nitric oxide (NO) is involved in cutaneous active vasodilation during hyperthermia in humans is unclear. We tested for a role of NO in this process during heat stress (water-perfused suits) in seven healthy subjects. Two forearm sites were instrumented with intradermal microdialysis probes. One site was perfused with the NO synthase inhibitor N(G)-nitro-L-arginine methyl ester (L-NAME) dissolved in Ringer solution to abolish NO production. The other site was perfused with Ringer solution only. At those sites, skin blood flow (laser-Doppler flowmetry) and sweat rate were simultaneously and continuously monitored. Cutaneous vascular conductance, calculated from laserDoppler fiowmetry and mean arterial pressure, was normalized to maximal levels as achieved by perfusion with the NO donor nitroprusside through the microdialysis probes. Under normothermic conditions, L-NAME did not significantly reduce cutaneous vascular conductance. During hyperthermia, with skin temperature held at 38-38.5°C, internal temperature rose from 36.66 ± 0.10 to 37.34 ± 0.06°C (P < 0.01). Cutaneous vascular conductance at untreated sites increased from 12 ± 2 to 44 ± 5% of maximum, but only rose from 13 ± 2 to 30 ± 5% of maximum at L-NAME-treated sites (P < 0.05 between sites) during heat stress. L-NAME had no effect on sweat rate (P > 0.05). Thus cutaneous active vasodilation requires functional NO synthase to achieve full expression.

Original languageEnglish (US)
Pages (from-to)824-829
Number of pages6
JournalJournal of Applied Physiology
Volume85
Issue number3
StatePublished - Sep 1998

Fingerprint

Vasodilation
Nitric Oxide
Hot Temperature
NG-Nitroarginine Methyl Ester
Skin
Microdialysis
Nitric Oxide Synthase
Blood Vessels
Fever
Laser-Doppler Flowmetry
Nitric Oxide Donors
Skin Temperature
Sweat
Nitroprusside
Dehydration
Forearm
Healthy Volunteers
Arterial Pressure
Perfusion
Temperature

Keywords

  • Laser-Doppler flowmetry
  • Microdialysis
  • Skin blood flow

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Kellogg, D. L., Crandall, C. G., Liu, Y., Charkoudian, N., & Johnson, J. M. (1998). Nitric oxide and cutaneous active vasodilation during heat stress in humans. Journal of Applied Physiology, 85(3), 824-829.

Nitric oxide and cutaneous active vasodilation during heat stress in humans. / Kellogg, D. L.; Crandall, C. G.; Liu, Y.; Charkoudian, N.; Johnson, J. M.

In: Journal of Applied Physiology, Vol. 85, No. 3, 09.1998, p. 824-829.

Research output: Contribution to journalArticle

Kellogg, DL, Crandall, CG, Liu, Y, Charkoudian, N & Johnson, JM 1998, 'Nitric oxide and cutaneous active vasodilation during heat stress in humans', Journal of Applied Physiology, vol. 85, no. 3, pp. 824-829.
Kellogg, D. L. ; Crandall, C. G. ; Liu, Y. ; Charkoudian, N. ; Johnson, J. M. / Nitric oxide and cutaneous active vasodilation during heat stress in humans. In: Journal of Applied Physiology. 1998 ; Vol. 85, No. 3. pp. 824-829.
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