Nitric oxide attenuates and xanthine oxidase exaggerates lung damage- induced gut injury

Eric C. Brooks, Nancy N. Mahr, Zivotije Radisavljevic, Eugene D. Jacobson, Lance S. Terada

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Aspirated gastric contents can evoke multiorgan failure. We hypothesized that secondary intestinal epithelial dysfunction after lung damage would be mediated by xanthine oxidase (XO) and antagonized by endogenous gut nitric oxide (NO). Isosmotic saline or HCl solutions were instilled intratracheally in anesthetized rats, and intestinal injury was assessed 190 min later by measuring the blood-to-lumen clearance of 51Cr-labeled EDTA (51Cr-EDTA clearance) and gut wall neutrophil population density. Intratracheal HCl increased 51Cr-EDTA clearance, and this transepithelial leak was attenuated by either systemic L-arginine or intraluminal NO and by chronic dietary pretreatment with allopurinol or sodium tungstate. Conversely, lung damage- induced gut leak was exaggerated by NO synthase inhibition or intravenous XO administration. Intratracheal HCl also increased intestinal wall neutrophil density and myeloperoxide activity. We conclude that two enzymatic systems involved in remote gut barrier dysfunction after endobronchial acidification are XO as mediator and NO synthase as antagonist.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume272
Issue number4 35-4
StatePublished - Apr 1997

Fingerprint

Xanthine Oxidase
Edetic Acid
Nitric Oxide
Nitric Oxide Synthase
Lung
Wounds and Injuries
Neutrophils
Gastrointestinal Contents
Allopurinol
Population Density
Arginine

Keywords

  • intestinal permeability
  • L-arginine
  • neutrophils
  • nitric oxide synthase inhibition

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology
  • Physiology (medical)

Cite this

Nitric oxide attenuates and xanthine oxidase exaggerates lung damage- induced gut injury. / Brooks, Eric C.; Mahr, Nancy N.; Radisavljevic, Zivotije; Jacobson, Eugene D.; Terada, Lance S.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 272, No. 4 35-4, 04.1997.

Research output: Contribution to journalArticle

Brooks, Eric C. ; Mahr, Nancy N. ; Radisavljevic, Zivotije ; Jacobson, Eugene D. ; Terada, Lance S. / Nitric oxide attenuates and xanthine oxidase exaggerates lung damage- induced gut injury. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 1997 ; Vol. 272, No. 4 35-4.
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