TY - JOUR
T1 - Nitric oxide inhibits IFNγ-induced increases in CIITA mRNA abundance and activation of CIITA dependent genesclass II MHC, Ii and H-2M
AU - Kielar, Mariusz L.
AU - Sicher, Stanley C.
AU - Penfield, Jeffery G.
AU - Jeyarajah, D. Rohan
AU - Lu, Christopher Y.
N1 - Funding Information:
Acknowledgments—C. Y. Lu was supported by the March of Dimes, the Welch Foundation, and NIH grants RO1-HD242792, RO1-DK 54304, and KO4-HD00862. M. L. Kielar was supported by an Individual Research Service Award from the NIH and by the American Heart Association. J. Penfield supported by a fellowship grant from the National Kidney Foundation.
PY - 2000
Y1 - 2000
N2 - Background: Nitric oxide (NO) has been recently implicated as a powerful inhibitor of immune responses during allograft rejection, and some autoimmune and infectious diseases. We previously showed that one potential regulatory effect of NO is inhibition of IFNγ-stimulated expression of Class II MHC on macrophages. Activation of this gene is mediated by the 'Class II TransActivator' (CIITA). We now ask whether NO inhibits CIITA and thus the family of genes regulated by CIITA-Class II MHC, Ii, and H-2M. The latter two genes participate in antigen processing and formation of the cell-surface peptide-Class II MHC complex. Methods: Murine macrophages - both peritoneal macrophages and the RAW264.7 macrophage line - were stimulated in vitro with IFNγ. NO production was measured by the Greiss reagent. Transcription of Class II MHC was measured by nuclear run-on assay, mRNA abundance of Class II MHC, Ii, H-2M, and CIITA was measured by Northern blotting and RT-PCR. Results: NO inhibits IFNγ-induced increases in the abundance and transcription of the Class II MHC Ab gene. The increases in MRNA abundance of CIITA, Ii, and H-2M are also inhibited. As a control, we found that NO did not inhibit LPS-induce increases in TNFα MRNA abundance. Conclusions: NO inhibits IFNγ-induced increases in CIITA, and thus inhibits the CIITA-regulated genes: Class II MHC, Ii, and H- 2M Early during rejection, NO production by macrophages may result after stimulation by IFNγ produced by CD4+ T cells, and be an effector of allograft damage. High concentrations of NO may then act as a feedback inhibitor which decreases antigen presentation by macrophages and thus decreases CD4 T cell activation.
AB - Background: Nitric oxide (NO) has been recently implicated as a powerful inhibitor of immune responses during allograft rejection, and some autoimmune and infectious diseases. We previously showed that one potential regulatory effect of NO is inhibition of IFNγ-stimulated expression of Class II MHC on macrophages. Activation of this gene is mediated by the 'Class II TransActivator' (CIITA). We now ask whether NO inhibits CIITA and thus the family of genes regulated by CIITA-Class II MHC, Ii, and H-2M. The latter two genes participate in antigen processing and formation of the cell-surface peptide-Class II MHC complex. Methods: Murine macrophages - both peritoneal macrophages and the RAW264.7 macrophage line - were stimulated in vitro with IFNγ. NO production was measured by the Greiss reagent. Transcription of Class II MHC was measured by nuclear run-on assay, mRNA abundance of Class II MHC, Ii, H-2M, and CIITA was measured by Northern blotting and RT-PCR. Results: NO inhibits IFNγ-induced increases in the abundance and transcription of the Class II MHC Ab gene. The increases in MRNA abundance of CIITA, Ii, and H-2M are also inhibited. As a control, we found that NO did not inhibit LPS-induce increases in TNFα MRNA abundance. Conclusions: NO inhibits IFNγ-induced increases in CIITA, and thus inhibits the CIITA-regulated genes: Class II MHC, Ii, and H- 2M Early during rejection, NO production by macrophages may result after stimulation by IFNγ produced by CD4+ T cells, and be an effector of allograft damage. High concentrations of NO may then act as a feedback inhibitor which decreases antigen presentation by macrophages and thus decreases CD4 T cell activation.
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U2 - 10.1023/A:1007012128392
DO - 10.1023/A:1007012128392
M3 - Article
C2 - 10921507
AN - SCOPUS:2142659972
SN - 0360-3997
VL - 24
SP - 431
EP - 445
JO - Inflammation
JF - Inflammation
IS - 5
ER -