NMDA receptors mediate hypoxic spine loss in cultured neurons

M. Josh Hasbani, N. M. Viquez, M. P. Goldberg

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

We examined the pharmacology of dendritic morphologic changes in cultured cortical neurons exposed to sublethal oxygen-glucose deprivation (OGD). Confocal analysis of Dillabeled neurons demonstrated transient dendritic swelling and spine loss after OGD. These morphological changes were reproduced by direct application of NMDA, kainate, veratridine, ionomycyin, and gramicidin, but not KCI. Blockade of voltage-gated sodium or calcium channels did not prevent OGD-induced dendritic spine loss. In contrast, the NMDA receptor antagonist, MK-801, fully prevented these changes. An AMPA/kainate receptor antagonist, NBQX, had no effect by itself but reduced spine loss when added to MK-801. While alterations in dendrite morphology may be triggered by activation of disparate ion channels, rapid spine loss in hypoxic cortical neurons is mediated preferentially through activation of the NMDA subtype glutamate receptor.

Original languageEnglish (US)
Pages (from-to)2731-2735
Number of pages5
JournalNeuroReport
Volume12
Issue number12
DOIs
StatePublished - Aug 28 2001

Keywords

  • Cell culture
  • Dendrite
  • Excitotoxicity
  • Glutamate
  • Hypoxia
  • Spine

ASJC Scopus subject areas

  • Neuroscience(all)

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