NMR indirect detection of glutamate to measure citric acid cycle flux in the isolated perfused mouse heart

Shawn C. Burgess; Evelyn E. Babcock; F. MarkH Jeffrey; A. Dean Sherry; Craig R. Malloy

doi:10.1016/S0014-5793(01)02799-5

NMR indirect detection of glutamate to measure citric acid cycle flux in the isolated perfused mouse heart

Shawn C. Burgess, Evelyn E. Babcock, F. MarkH Jeffrey, A. Dean Sherry, Craig R. Malloy

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

¹³C-edited proton nuclear magnetic resonance (NMR) spectroscopy was used to follow enrichment of glutamate C3 and C4 with a temporal resolution of ∼20 s in mouse hearts perfused with ¹³C-enriched substrates. A fit of the NMR data to a kinetic model of the tricarboxylic acid (TCA) cycle and related exchange reactions yielded TCA cycle (V_tca) and exchange (V_x) fluxes between α-ketoglutarate and glutamate. These fluxes were substrate-dependent and decreased in the order acetate (V_tca = 14.1 μmol g^-1 min^-1; V_x = 26.5 μmol g^-1 min^-1) > octanoate (V_tca = 6.0 μmol g^-1 min^-1; V_x = 16.1 μmol g^-1 min^-1)lactate (V_tca = 4.2 μmol g^-1 min^-1; V_x = 6.3 μmol g^-1 min^-1).

Original language	English (US)
Pages (from-to)	163-167
Number of pages	5
Journal	FEBS Letters
Volume	505
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(01)02799-5
State	Published - Sep 7 2001

Keywords

Cardiac metabolism
H nuclear magnetic resonance
Indirect detection
Tricarboxylic acid cycle kinetics

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/S0014-5793(01)02799-5

Cite this

@article{e16459a7079a45a18e2068428b6423f1,

title = "NMR indirect detection of glutamate to measure citric acid cycle flux in the isolated perfused mouse heart",

abstract = "13C-edited proton nuclear magnetic resonance (NMR) spectroscopy was used to follow enrichment of glutamate C3 and C4 with a temporal resolution of ∼20 s in mouse hearts perfused with 13C-enriched substrates. A fit of the NMR data to a kinetic model of the tricarboxylic acid (TCA) cycle and related exchange reactions yielded TCA cycle (Vtca) and exchange (Vx) fluxes between α-ketoglutarate and glutamate. These fluxes were substrate-dependent and decreased in the order acetate (Vtca = 14.1 μmol g-1 min-1; Vx = 26.5 μmol g-1 min-1) > octanoate (Vtca = 6.0 μmol g-1 min-1; Vx = 16.1 μmol g-1 min-1)lactate (Vtca = 4.2 μmol g-1 min-1; Vx = 6.3 μmol g-1 min-1).",

keywords = "Cardiac metabolism, H nuclear magnetic resonance, Indirect detection, Tricarboxylic acid cycle kinetics",

author = "Burgess, {Shawn C.} and Babcock, {Evelyn E.} and Jeffrey, {F. MarkH} and Sherry, {A. Dean} and Malloy, {Craig R.}",

note = "Funding Information: Special thanks to Ms. Angela Richman for her technical assistance with the heart perfusions. This work was supported by Grants from the National Institutes of Health (HL-34557 and RR-02584).",

year = "2001",

month = sep,

day = "7",

doi = "10.1016/S0014-5793(01)02799-5",

language = "English (US)",

volume = "505",

pages = "163--167",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - NMR indirect detection of glutamate to measure citric acid cycle flux in the isolated perfused mouse heart

AU - Burgess, Shawn C.

AU - Babcock, Evelyn E.

AU - Jeffrey, F. MarkH

AU - Sherry, A. Dean

AU - Malloy, Craig R.

N1 - Funding Information: Special thanks to Ms. Angela Richman for her technical assistance with the heart perfusions. This work was supported by Grants from the National Institutes of Health (HL-34557 and RR-02584).

PY - 2001/9/7

Y1 - 2001/9/7

N2 - 13C-edited proton nuclear magnetic resonance (NMR) spectroscopy was used to follow enrichment of glutamate C3 and C4 with a temporal resolution of ∼20 s in mouse hearts perfused with 13C-enriched substrates. A fit of the NMR data to a kinetic model of the tricarboxylic acid (TCA) cycle and related exchange reactions yielded TCA cycle (Vtca) and exchange (Vx) fluxes between α-ketoglutarate and glutamate. These fluxes were substrate-dependent and decreased in the order acetate (Vtca = 14.1 μmol g-1 min-1; Vx = 26.5 μmol g-1 min-1) > octanoate (Vtca = 6.0 μmol g-1 min-1; Vx = 16.1 μmol g-1 min-1)lactate (Vtca = 4.2 μmol g-1 min-1; Vx = 6.3 μmol g-1 min-1).

AB - 13C-edited proton nuclear magnetic resonance (NMR) spectroscopy was used to follow enrichment of glutamate C3 and C4 with a temporal resolution of ∼20 s in mouse hearts perfused with 13C-enriched substrates. A fit of the NMR data to a kinetic model of the tricarboxylic acid (TCA) cycle and related exchange reactions yielded TCA cycle (Vtca) and exchange (Vx) fluxes between α-ketoglutarate and glutamate. These fluxes were substrate-dependent and decreased in the order acetate (Vtca = 14.1 μmol g-1 min-1; Vx = 26.5 μmol g-1 min-1) > octanoate (Vtca = 6.0 μmol g-1 min-1; Vx = 16.1 μmol g-1 min-1)lactate (Vtca = 4.2 μmol g-1 min-1; Vx = 6.3 μmol g-1 min-1).

KW - Cardiac metabolism

KW - H nuclear magnetic resonance

KW - Indirect detection

KW - Tricarboxylic acid cycle kinetics

UR - http://www.scopus.com/inward/record.url?scp=0035823277&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035823277&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(01)02799-5

DO - 10.1016/S0014-5793(01)02799-5

M3 - Article

C2 - 11557062

AN - SCOPUS:0035823277

SN - 0014-5793

VL - 505

SP - 163

EP - 167

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

NMR indirect detection of glutamate to measure citric acid cycle flux in the isolated perfused mouse heart

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this