NMR visualization of free asparagine in potato tissue using adduct formation with [13C]formaldehyde

Ralph P. Mason; Jeremy K M Sanders; Michael J. Gidley

doi:10.1016/S0031-9422(00)81210-8

NMR visualization of free asparagine in potato tissue using adduct formation with [¹³C]formaldehyde

Ralph P. Mason, Jeremy K M Sanders, Michael J. Gidley

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The free asparagine in potato (Solanum tuberosum) tuber tissue has been observed by ¹³C NMR, using labelled formaldehyde as a marker; formaldehyde-asparagine adduct formation is specific and leads to characteristic ¹³C resonances. In addition, metabolism of formaldehyde to methanol and formate by potato tissue has been observed by ¹³C and deuterium NMR. Metabolism of formaldehyde-d₂ leads to a 3:1 mixture of CD₃OH and CD₂HOH.

Original language	English (US)
Pages (from-to)	1567-1571
Number of pages	5
Journal	Phytochemistry
Volume	25
Issue number	7
DOIs	https://doi.org/10.1016/S0031-9422(00)81210-8
State	Published - 1986

Keywords

C NMR
Solanaceae
Solanum tuberosum
asparagine
deuterium NMR
formaldehyde-metabolism.
potato

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Plant Science
Horticulture

Access to Document

10.1016/S0031-9422(00)81210-8

Cite this

@article{152bf6cb89c74daca5eb435094a1c725,

title = "NMR visualization of free asparagine in potato tissue using adduct formation with [13C]formaldehyde",

abstract = "The free asparagine in potato (Solanum tuberosum) tuber tissue has been observed by 13C NMR, using labelled formaldehyde as a marker; formaldehyde-asparagine adduct formation is specific and leads to characteristic 13C resonances. In addition, metabolism of formaldehyde to methanol and formate by potato tissue has been observed by 13C and deuterium NMR. Metabolism of formaldehyde-d2 leads to a 3:1 mixture of CD3OH and CD2HOH.",

keywords = "C NMR, Solanaceae, Solanum tuberosum, asparagine, deuterium NMR, formaldehyde-metabolism., potato",

author = "Mason, {Ralph P.} and Sanders, {Jeremy K M} and Gidley, {Michael J.}",

year = "1986",

doi = "10.1016/S0031-9422(00)81210-8",

language = "English (US)",

volume = "25",

pages = "1567--1571",

journal = "Phytochemistry",

issn = "0031-9422",

publisher = "Elsevier Limited",

number = "7",

}

TY - JOUR

T1 - NMR visualization of free asparagine in potato tissue using adduct formation with [13C]formaldehyde

AU - Mason, Ralph P.

AU - Sanders, Jeremy K M

AU - Gidley, Michael J.

PY - 1986

Y1 - 1986

N2 - The free asparagine in potato (Solanum tuberosum) tuber tissue has been observed by 13C NMR, using labelled formaldehyde as a marker; formaldehyde-asparagine adduct formation is specific and leads to characteristic 13C resonances. In addition, metabolism of formaldehyde to methanol and formate by potato tissue has been observed by 13C and deuterium NMR. Metabolism of formaldehyde-d2 leads to a 3:1 mixture of CD3OH and CD2HOH.

AB - The free asparagine in potato (Solanum tuberosum) tuber tissue has been observed by 13C NMR, using labelled formaldehyde as a marker; formaldehyde-asparagine adduct formation is specific and leads to characteristic 13C resonances. In addition, metabolism of formaldehyde to methanol and formate by potato tissue has been observed by 13C and deuterium NMR. Metabolism of formaldehyde-d2 leads to a 3:1 mixture of CD3OH and CD2HOH.

KW - C NMR

KW - Solanaceae

KW - Solanum tuberosum

KW - asparagine

KW - deuterium NMR

KW - formaldehyde-metabolism.

KW - potato

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U2 - 10.1016/S0031-9422(00)81210-8

DO - 10.1016/S0031-9422(00)81210-8

M3 - Article

AN - SCOPUS:0001662995

SN - 0031-9422

VL - 25

SP - 1567

EP - 1571

JO - Phytochemistry

JF - Phytochemistry

IS - 7

ER -