NMR visualization of free asparagine in potato tissue using adduct formation with [13C]formaldehyde

Ralph P. Mason, Jeremy K M Sanders, Michael J. Gidley

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The free asparagine in potato (Solanum tuberosum) tuber tissue has been observed by 13C NMR, using labelled formaldehyde as a marker; formaldehyde-asparagine adduct formation is specific and leads to characteristic 13C resonances. In addition, metabolism of formaldehyde to methanol and formate by potato tissue has been observed by 13C and deuterium NMR. Metabolism of formaldehyde-d2 leads to a 3:1 mixture of CD3OH and CD2HOH.

Original languageEnglish (US)
Pages (from-to)1567-1571
Number of pages5
JournalPhytochemistry
Volume25
Issue number7
DOIs
StatePublished - 1986

Keywords

  • C NMR
  • Solanaceae
  • Solanum tuberosum
  • asparagine
  • deuterium NMR
  • formaldehyde-metabolism.
  • potato

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture

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