No phenotype associated with established lipopolysaccharide model for cerebral palsy

Sarah H. Poggi, Jane Park, Laura Toso, Daniel Abebe, Robin Roberson, Jade E. Woodard, Catherine Y. Spong

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Objective: Cerebral palsy (CP) is associated with childhood spasticity, seizures, and paralysis. Oligodendrocyte damage resulting in periventicular leukomalacia (PVL) in the developing brain has been implicated. Animal models of CP have used prenatal hypoxia and infection with histopathology of PVL as the end point. To evaluate whether this histologic end point is associated with a CP phenotype, we reproduced a lipopolysaccharide (LPS) model for PVL,1 and evaluated developmental, behavioral, and motor outcomes. Study design: On gestational day 15, Fischer 344 rats were intracervically injected with. 1 mg/kg LPS (n = 5) or saline (n = 4). After delivery, evaluation for developmental milestones was performed on days 1 to 21 (LPS = 45; control = 30 pups). Males were also tested at 2.5 months using open-field, rotarod, and anxiety tests. On day 21, 2 pups/litter were perfused for immunohistochemistry, and stained with 2 oligodendrocyte antibodies: 2′, d'-cyclic nucleotide phosphodiesterase (CNP), and myelin proteolipid protein (PLP) with relative densities of staining assessed using NIH Image software. Statistical analysis included Mann-Whitney U and analysis of variance (ANOVA). Results: LPS pups demonstrated decreased CNP (P =. 04) and PLP (P =. 06) staining, replicating the model. There was no difference seen in neonatal weight, righting, negative geotaxis, cliff aversion, rooting, forelimb grasp, audio startle, air righting, eye opening, and activity. Surprisingly, LPS-exposed neonatal rats mastered forelimb placement (P <. 01) and surface righting (P =. 02) earlier than control rats. There were no differences between adult groups in open field distance traveled (P =. 8), open-field locomotion time (P =. 6), rotarod (P =. 6), or anxiety (P =. 7). Conclusion: Histologic evidence of white matter damage can be replicated using an LPS model for intrauterine inflammation. Significant phenotypic differences consistent with the motor and cognitive damage sequelae of such lesions (ie, CP) were not demonstrated. When evaluating animal models, it is important to assess not only biochemical markers for human disease, but also clinically relevant phenotypes.

Original languageEnglish (US)
Pages (from-to)727-733
Number of pages7
JournalAmerican journal of obstetrics and gynecology
Volume192
Issue number3
DOIs
StatePublished - Mar 2005
Externally publishedYes

Fingerprint

Cerebral Palsy
Lipopolysaccharides
Phenotype
Forelimb
Cyclic Nucleotides
Oligodendroglia
Phosphoric Diester Hydrolases
Anxiety
Myelin Proteolipid Protein
Animal Models
Rotarod Performance Test
Staining and Labeling
Proteolipids
Specific Gravity
Inbred F344 Rats
Hand Strength
Locomotion
Paralysis
Analysis of Variance
Seizures

Keywords

  • Cerebral palsy
  • Lipopolysaccharide
  • Periventricular leukomalacia
  • Rat

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

No phenotype associated with established lipopolysaccharide model for cerebral palsy. / Poggi, Sarah H.; Park, Jane; Toso, Laura; Abebe, Daniel; Roberson, Robin; Woodard, Jade E.; Spong, Catherine Y.

In: American journal of obstetrics and gynecology, Vol. 192, No. 3, 03.2005, p. 727-733.

Research output: Contribution to journalArticle

Poggi, Sarah H. ; Park, Jane ; Toso, Laura ; Abebe, Daniel ; Roberson, Robin ; Woodard, Jade E. ; Spong, Catherine Y. / No phenotype associated with established lipopolysaccharide model for cerebral palsy. In: American journal of obstetrics and gynecology. 2005 ; Vol. 192, No. 3. pp. 727-733.
@article{9778de86350a4156a7c7a3bc2555410e,
title = "No phenotype associated with established lipopolysaccharide model for cerebral palsy",
abstract = "Objective: Cerebral palsy (CP) is associated with childhood spasticity, seizures, and paralysis. Oligodendrocyte damage resulting in periventicular leukomalacia (PVL) in the developing brain has been implicated. Animal models of CP have used prenatal hypoxia and infection with histopathology of PVL as the end point. To evaluate whether this histologic end point is associated with a CP phenotype, we reproduced a lipopolysaccharide (LPS) model for PVL,1 and evaluated developmental, behavioral, and motor outcomes. Study design: On gestational day 15, Fischer 344 rats were intracervically injected with. 1 mg/kg LPS (n = 5) or saline (n = 4). After delivery, evaluation for developmental milestones was performed on days 1 to 21 (LPS = 45; control = 30 pups). Males were also tested at 2.5 months using open-field, rotarod, and anxiety tests. On day 21, 2 pups/litter were perfused for immunohistochemistry, and stained with 2 oligodendrocyte antibodies: 2′, d'-cyclic nucleotide phosphodiesterase (CNP), and myelin proteolipid protein (PLP) with relative densities of staining assessed using NIH Image software. Statistical analysis included Mann-Whitney U and analysis of variance (ANOVA). Results: LPS pups demonstrated decreased CNP (P =. 04) and PLP (P =. 06) staining, replicating the model. There was no difference seen in neonatal weight, righting, negative geotaxis, cliff aversion, rooting, forelimb grasp, audio startle, air righting, eye opening, and activity. Surprisingly, LPS-exposed neonatal rats mastered forelimb placement (P <. 01) and surface righting (P =. 02) earlier than control rats. There were no differences between adult groups in open field distance traveled (P =. 8), open-field locomotion time (P =. 6), rotarod (P =. 6), or anxiety (P =. 7). Conclusion: Histologic evidence of white matter damage can be replicated using an LPS model for intrauterine inflammation. Significant phenotypic differences consistent with the motor and cognitive damage sequelae of such lesions (ie, CP) were not demonstrated. When evaluating animal models, it is important to assess not only biochemical markers for human disease, but also clinically relevant phenotypes.",
keywords = "Cerebral palsy, Lipopolysaccharide, Periventricular leukomalacia, Rat",
author = "Poggi, {Sarah H.} and Jane Park and Laura Toso and Daniel Abebe and Robin Roberson and Woodard, {Jade E.} and Spong, {Catherine Y.}",
year = "2005",
month = "3",
doi = "10.1016/j.ajog.2004.12.053",
language = "English (US)",
volume = "192",
pages = "727--733",
journal = "American Journal of Obstetrics and Gynecology",
issn = "0002-9378",
publisher = "Mosby Inc.",
number = "3",

}

TY - JOUR

T1 - No phenotype associated with established lipopolysaccharide model for cerebral palsy

AU - Poggi, Sarah H.

AU - Park, Jane

AU - Toso, Laura

AU - Abebe, Daniel

AU - Roberson, Robin

AU - Woodard, Jade E.

AU - Spong, Catherine Y.

PY - 2005/3

Y1 - 2005/3

N2 - Objective: Cerebral palsy (CP) is associated with childhood spasticity, seizures, and paralysis. Oligodendrocyte damage resulting in periventicular leukomalacia (PVL) in the developing brain has been implicated. Animal models of CP have used prenatal hypoxia and infection with histopathology of PVL as the end point. To evaluate whether this histologic end point is associated with a CP phenotype, we reproduced a lipopolysaccharide (LPS) model for PVL,1 and evaluated developmental, behavioral, and motor outcomes. Study design: On gestational day 15, Fischer 344 rats were intracervically injected with. 1 mg/kg LPS (n = 5) or saline (n = 4). After delivery, evaluation for developmental milestones was performed on days 1 to 21 (LPS = 45; control = 30 pups). Males were also tested at 2.5 months using open-field, rotarod, and anxiety tests. On day 21, 2 pups/litter were perfused for immunohistochemistry, and stained with 2 oligodendrocyte antibodies: 2′, d'-cyclic nucleotide phosphodiesterase (CNP), and myelin proteolipid protein (PLP) with relative densities of staining assessed using NIH Image software. Statistical analysis included Mann-Whitney U and analysis of variance (ANOVA). Results: LPS pups demonstrated decreased CNP (P =. 04) and PLP (P =. 06) staining, replicating the model. There was no difference seen in neonatal weight, righting, negative geotaxis, cliff aversion, rooting, forelimb grasp, audio startle, air righting, eye opening, and activity. Surprisingly, LPS-exposed neonatal rats mastered forelimb placement (P <. 01) and surface righting (P =. 02) earlier than control rats. There were no differences between adult groups in open field distance traveled (P =. 8), open-field locomotion time (P =. 6), rotarod (P =. 6), or anxiety (P =. 7). Conclusion: Histologic evidence of white matter damage can be replicated using an LPS model for intrauterine inflammation. Significant phenotypic differences consistent with the motor and cognitive damage sequelae of such lesions (ie, CP) were not demonstrated. When evaluating animal models, it is important to assess not only biochemical markers for human disease, but also clinically relevant phenotypes.

AB - Objective: Cerebral palsy (CP) is associated with childhood spasticity, seizures, and paralysis. Oligodendrocyte damage resulting in periventicular leukomalacia (PVL) in the developing brain has been implicated. Animal models of CP have used prenatal hypoxia and infection with histopathology of PVL as the end point. To evaluate whether this histologic end point is associated with a CP phenotype, we reproduced a lipopolysaccharide (LPS) model for PVL,1 and evaluated developmental, behavioral, and motor outcomes. Study design: On gestational day 15, Fischer 344 rats were intracervically injected with. 1 mg/kg LPS (n = 5) or saline (n = 4). After delivery, evaluation for developmental milestones was performed on days 1 to 21 (LPS = 45; control = 30 pups). Males were also tested at 2.5 months using open-field, rotarod, and anxiety tests. On day 21, 2 pups/litter were perfused for immunohistochemistry, and stained with 2 oligodendrocyte antibodies: 2′, d'-cyclic nucleotide phosphodiesterase (CNP), and myelin proteolipid protein (PLP) with relative densities of staining assessed using NIH Image software. Statistical analysis included Mann-Whitney U and analysis of variance (ANOVA). Results: LPS pups demonstrated decreased CNP (P =. 04) and PLP (P =. 06) staining, replicating the model. There was no difference seen in neonatal weight, righting, negative geotaxis, cliff aversion, rooting, forelimb grasp, audio startle, air righting, eye opening, and activity. Surprisingly, LPS-exposed neonatal rats mastered forelimb placement (P <. 01) and surface righting (P =. 02) earlier than control rats. There were no differences between adult groups in open field distance traveled (P =. 8), open-field locomotion time (P =. 6), rotarod (P =. 6), or anxiety (P =. 7). Conclusion: Histologic evidence of white matter damage can be replicated using an LPS model for intrauterine inflammation. Significant phenotypic differences consistent with the motor and cognitive damage sequelae of such lesions (ie, CP) were not demonstrated. When evaluating animal models, it is important to assess not only biochemical markers for human disease, but also clinically relevant phenotypes.

KW - Cerebral palsy

KW - Lipopolysaccharide

KW - Periventricular leukomalacia

KW - Rat

UR - http://www.scopus.com/inward/record.url?scp=14644400486&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14644400486&partnerID=8YFLogxK

U2 - 10.1016/j.ajog.2004.12.053

DO - 10.1016/j.ajog.2004.12.053

M3 - Article

C2 - 15746664

AN - SCOPUS:14644400486

VL - 192

SP - 727

EP - 733

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

SN - 0002-9378

IS - 3

ER -