Nocturnin, a deadenylase in Xenopus laevis retina: A mechanism for posttranscriptional control of circadian-related mRNA

Julie E. Baggs, Carla B. Green

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

Background: Different types of regulation are utilized to produce a robust circadian clock, including regulation at the transcriptional, posttranscriptional, and translational levels. A screen for rhythmic messages that may be involved in such circadian control identified nocturnin, a novel gene that displays high-amplitude circadian expression in the Xenopus laevis retina, with peak mRNA levels in the early night. Expression of nocturnin mRNA is confined to the clock-containing photoreceptor cell layer within the retina. Results: In these studies, we show that nocturnin removes the poly(A) tail from a synthetic RNA substrate in a process known as deadenylation. Nocturnin nuclease activity is magnesium dependent, as the addition of EDTA or mutation of the residue predicted to bind magnesium disrupts deadenylation. Substrate preference studies show that nocturnin is an exonuclease that specifically degrades the 3′ poly(A) tail. While nocturnin is rhythmically expressed in the cytoplasm of the retinal photoreceptor cells, the only other described vertebrate deadenylase, PARN, is constitutively present in most retinal cells, including the photoreceptors. Conclusions: The distinct spatial and temporal expression of nocturnin and PARN suggests that there may be specific mRNA targets of each deadenylase. Since deadenylation regulates mRNA decay and/or translational silencing, we propose that nocturnin deadenylates clock-related transcripts in a novel mechanism for posttranscriptional regulation in the circadian clock or its outputs.

Original languageEnglish (US)
Pages (from-to)189-198
Number of pages10
JournalCurrent Biology
Volume13
Issue number3
DOIs
StatePublished - Feb 2003

Fingerprint

Xenopus laevis
photoreceptors
retina
Retina
messenger RNA
circadian rhythm
Messenger RNA
magnesium
Clocks
nucleases
cells
Vertebrate Photoreceptor Cells
Circadian Clocks
cytoplasm
deterioration
vertebrates
RNA
Magnesium
mutation
Cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Nocturnin, a deadenylase in Xenopus laevis retina : A mechanism for posttranscriptional control of circadian-related mRNA. / Baggs, Julie E.; Green, Carla B.

In: Current Biology, Vol. 13, No. 3, 02.2003, p. 189-198.

Research output: Contribution to journalArticle

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