Nocturnin regulates circadian trafficking of dietary lipid in intestinal enterocytes

Nicholas Douris, Shihoko Kojima, Xiaoyue Pan, Alexandra F. Lerch-Gaggl, Son Q. Duong, M. Mahmood Hussain, Carla B. Green

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Background: Efficient metabolic function in mammals depends on the circadian clock, which drives temporal regulation of metabolic processes. Nocturnin is a clock-regulated deadenylase that controls its target mRNA expression posttranscriptionally through poly(A) tail removal. Mice lacking nocturnin (Noc -/- mice) are resistant to diet-induced obesity and hepatic steatosis yet are not hyperactive or hypophagic. Results: Here we show that nocturnin is expressed rhythmically in the small intestine and is induced by olive oil gavage and that the Noc -/- mice have reduced chylomicron transit into the plasma following the ingestion of dietary lipids. Genes involved in triglyceride synthesis and storage and chylomicron formation have altered expression, and large cytoplasmic lipid droplets accumulate in the apical domains of the Noc -/- enterocytes. The physiological significance of this deficit in absorption is clear because maintenance of Noc -/- mice on diets that challenge the chylomicron synthesis pathway result in significant reductions in body weight, whereas diets that bypass this pathway do not. Conclusions: Therefore, we propose that nocturnin plays an important role in the trafficking of dietary lipid in the intestinal enterocytes by optimizing efficient absorption of lipids.

Original languageEnglish (US)
Pages (from-to)1347-1355
Number of pages9
JournalCurrent Biology
Volume21
Issue number16
DOIs
StatePublished - Aug 23 2011

Fingerprint

Enterocytes
enterocytes
Chylomicrons
chylomicrons
dietary fat
Nutrition
Lipids
mice
Diet
Clocks
diet
Messenger RNA
Circadian Clocks
Mammals
synthesis
fatty liver
lipids
messenger RNA
olive oil
circadian rhythm

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Douris, N., Kojima, S., Pan, X., Lerch-Gaggl, A. F., Duong, S. Q., Hussain, M. M., & Green, C. B. (2011). Nocturnin regulates circadian trafficking of dietary lipid in intestinal enterocytes. Current Biology, 21(16), 1347-1355. https://doi.org/10.1016/j.cub.2011.07.018

Nocturnin regulates circadian trafficking of dietary lipid in intestinal enterocytes. / Douris, Nicholas; Kojima, Shihoko; Pan, Xiaoyue; Lerch-Gaggl, Alexandra F.; Duong, Son Q.; Hussain, M. Mahmood; Green, Carla B.

In: Current Biology, Vol. 21, No. 16, 23.08.2011, p. 1347-1355.

Research output: Contribution to journalArticle

Douris, N, Kojima, S, Pan, X, Lerch-Gaggl, AF, Duong, SQ, Hussain, MM & Green, CB 2011, 'Nocturnin regulates circadian trafficking of dietary lipid in intestinal enterocytes', Current Biology, vol. 21, no. 16, pp. 1347-1355. https://doi.org/10.1016/j.cub.2011.07.018
Douris N, Kojima S, Pan X, Lerch-Gaggl AF, Duong SQ, Hussain MM et al. Nocturnin regulates circadian trafficking of dietary lipid in intestinal enterocytes. Current Biology. 2011 Aug 23;21(16):1347-1355. https://doi.org/10.1016/j.cub.2011.07.018
Douris, Nicholas ; Kojima, Shihoko ; Pan, Xiaoyue ; Lerch-Gaggl, Alexandra F. ; Duong, Son Q. ; Hussain, M. Mahmood ; Green, Carla B. / Nocturnin regulates circadian trafficking of dietary lipid in intestinal enterocytes. In: Current Biology. 2011 ; Vol. 21, No. 16. pp. 1347-1355.
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