NOD-like receptors mediated activation of eosinophils interacting with bronchial epithelial cells: A link between innate immunity and allergic asthma

Chun Kwok Wong, Shuiqing Hu, Karen Ming Lam Leung, Jie Dong, Lan He, Yi Jun Chu, Ida Miu Ting Chu, Huai Na Qiu, Kelly Yan Ping Liu, Christopher Wai Kei Lam

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Key intracytosolic pattern recognition receptors of innate immunity against bacterial infections are nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs). We elucidated the NOD1 and NOD2-mediated activation of human eosinophils, the principal effector cells for allergic inflammation, upon interacting with human bronchial epithelial BEAS-2B cells in allergic asthma. Eosinophils constitutively expressed NOD1,2 but exhibited nonsignificant responses to release chemokines upon the stimulation by NOD1 ligand γ-D-glutamyl-meso-diaminopimelic acid (iE-DAP) and NOD2 ligand muramyl dipeptide (MDP). However, iE-DAP and MDP could significantly upregulate cell surface expression of CD18 and intercellular adhesion molecule (ICAM)-1 on eosinophils and ICAM-1 on BEAS-2B cells, as well as induce chemokines CCL2 and CXCL8 release in the coculture system (all P<0.05). Both eosinophils and BEAS-2B cells were the main source for CXCL8 and CCL2 release in the coculture system upon iE-DAP or MDP stimulation. Direct interaction between eosinophils and BEAS-2B cells is responsible for CCL2 release, and soluble mediators are implicated in CXCL8 release. ERK and NF-κB play regulatory roles for the expression of adhesion molecules and chemokines in coculture. Treatment with NOD1,2 ligand could induce the subepithelial fibrosis and significantly enhance the serum concentration of total IgE, chemokine CCL5 for eosinophils and T helper type 2 (Th2) cells and asthma Th2 cytokine IL-13 in bronchoalveolar lavage fluid of ovalbumin-sensitized allergic asthmatic mice (all P<0.05). This study provides further evidence of bacterial infection-mediated activation of NOD1,2 in triggering allergic asthma via the activation of eosinophils interacting with bronchial epithelial cells at inflammatory airway.

Original languageEnglish (US)
Pages (from-to)317-329
Number of pages13
JournalCellular and Molecular Immunology
Volume10
Issue number4
DOIs
StatePublished - Jul 1 2013

Fingerprint

Eosinophils
Innate Immunity
Asthma
Nucleotides
Epithelial Cells
Acetylmuramyl-Alanyl-Isoglutamine
Th2 Cells
Coculture Techniques
Intercellular Adhesion Molecule-1
Ligands
Chemokines
Bacterial Infections
Diaminopimelic Acid
Pattern Recognition Receptors
Chemokine CCL5
Interleukin-13
Chemokine CCL2
Ovalbumin
Bronchoalveolar Lavage Fluid
Interleukin-8

Keywords

  • allergy
  • bronchial epithelial cells
  • chemokines
  • eosinophils
  • signal transduction

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

NOD-like receptors mediated activation of eosinophils interacting with bronchial epithelial cells : A link between innate immunity and allergic asthma. / Wong, Chun Kwok; Hu, Shuiqing; Leung, Karen Ming Lam; Dong, Jie; He, Lan; Chu, Yi Jun; Chu, Ida Miu Ting; Qiu, Huai Na; Liu, Kelly Yan Ping; Lam, Christopher Wai Kei.

In: Cellular and Molecular Immunology, Vol. 10, No. 4, 01.07.2013, p. 317-329.

Research output: Contribution to journalArticle

Wong, Chun Kwok ; Hu, Shuiqing ; Leung, Karen Ming Lam ; Dong, Jie ; He, Lan ; Chu, Yi Jun ; Chu, Ida Miu Ting ; Qiu, Huai Na ; Liu, Kelly Yan Ping ; Lam, Christopher Wai Kei. / NOD-like receptors mediated activation of eosinophils interacting with bronchial epithelial cells : A link between innate immunity and allergic asthma. In: Cellular and Molecular Immunology. 2013 ; Vol. 10, No. 4. pp. 317-329.
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