Nogo enhances the adhesion of olfactory ensheathing cells and inhibits their migration

Zhida Su, Li Cao, Yanling Zhu, Xiujie Liu, Zhihui Huang, Aijun Huang, Cheng He

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The migration of olfactory ensheathing cells (OECs) is essential for pioneering the olfactory nerve pathway during development and for promoting axonal regeneration when implanted into the injured central nervous system (CNS). In the present study, recombinant Nogo-66 enhanced the adhesion of OECs and inhibited their migration. Using immunocytochemistry and western blot, we showed that the Nogo-66 receptor (NgR) was expressed on OECs. When NgR was released from the cell surface with phosphatidylinositol-specific phospholipase C or neutralized by NgR antibody, the effect of Nogo-66 on OEC adhesion and migration was markedly attenuated. Nogo-66 was found to promote the formation of focal adhesion in OECs and inhibited their membrane protrusion through the activation of RhoA. Furthermore, the co-culture migration assay demonstrated that OEC motility was significantly restricted by Nogo-A expressed on Cos7 cell membranes or oligodendrocytes. Moreover, treatment with anti-NgR antibody facilitated migration of implanted OECs in a spinal cord hemisection injury model. Taken together, we demonstrate, for the first time, that Nogo, a myelin-associated inhibitor of axon regeneration in the CNS, enhances the adhesion and inhibits the migration of OECs via NgR regulation of RhoA.

Original languageEnglish (US)
Pages (from-to)1877-1887
Number of pages11
JournalJournal of Cell Science
Volume120
Issue number11
DOIs
StatePublished - Jun 1 2007

Fingerprint

Cell Movement
Regeneration
Central Nervous System
Olfactory Nerve
Phosphoinositide Phospholipase C
Olfactory Pathways
Focal Adhesions
Antibodies
Oligodendroglia
Myelin Sheath
Coculture Techniques
Spinal Cord Injuries
Cell Adhesion
Axons
Western Blotting
Immunohistochemistry
Cell Membrane
Nogo Receptor 1
Membranes
Therapeutics

Keywords

  • Adhesion
  • Migration
  • Nogo
  • Olfactory ensheathing cells
  • RhoA
  • Spinal cord injury

ASJC Scopus subject areas

  • Cell Biology

Cite this

Su, Z., Cao, L., Zhu, Y., Liu, X., Huang, Z., Huang, A., & He, C. (2007). Nogo enhances the adhesion of olfactory ensheathing cells and inhibits their migration. Journal of Cell Science, 120(11), 1877-1887. https://doi.org/10.1242/jcs.03448

Nogo enhances the adhesion of olfactory ensheathing cells and inhibits their migration. / Su, Zhida; Cao, Li; Zhu, Yanling; Liu, Xiujie; Huang, Zhihui; Huang, Aijun; He, Cheng.

In: Journal of Cell Science, Vol. 120, No. 11, 01.06.2007, p. 1877-1887.

Research output: Contribution to journalArticle

Su, Z, Cao, L, Zhu, Y, Liu, X, Huang, Z, Huang, A & He, C 2007, 'Nogo enhances the adhesion of olfactory ensheathing cells and inhibits their migration', Journal of Cell Science, vol. 120, no. 11, pp. 1877-1887. https://doi.org/10.1242/jcs.03448
Su, Zhida ; Cao, Li ; Zhu, Yanling ; Liu, Xiujie ; Huang, Zhihui ; Huang, Aijun ; He, Cheng. / Nogo enhances the adhesion of olfactory ensheathing cells and inhibits their migration. In: Journal of Cell Science. 2007 ; Vol. 120, No. 11. pp. 1877-1887.
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