Noncytotoxic IgE mediated release of histamine and serotonin from murine mastocytoma cells

J. D. Taurog, G. R. Mendoza, W. Hook, R. P. Siraganian, H. Metzger

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

Cultured murine mastocytoma (AB-CBF1-MCT-1) cells were stimulated to release endogenous or incorporated histamine or serotonin by an IgE-mediated mechanism without loss of viability. Stimulation was achieved by incubation of the cells with rat IgE-anti-IgE, rat IgE-anti-light chain, fluoresceinated rat IgE-anti-fluorescein, IgE-enriched mouse anti-ovalbumin-ovalbumin, or covalently linked dimers of rat IgE, at doses similar to those optimal for normal peritoneal mast cells. Active cell metabolism and Ca++ were required to obtain release. Despite the latter, no dose of the calcium ionophore, A23187, could be found which caused release without concomitant cytotoxicity. Phosphatidylserine did not enhance release.

Original languageEnglish (US)
Pages (from-to)1757-1761
Number of pages5
JournalJournal of Immunology
Volume119
Issue number5
StatePublished - Dec 1 1977

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Taurog, J. D., Mendoza, G. R., Hook, W., Siraganian, R. P., & Metzger, H. (1977). Noncytotoxic IgE mediated release of histamine and serotonin from murine mastocytoma cells. Journal of Immunology, 119(5), 1757-1761.