Nonenzymatic Role for WRN in Preserving Nascent DNA Strands after Replication Stress

Fengtao Su, Shibani Mukherjee, Yanyong Yang, Eiichiro Mori, Souparno Bhattacharya, Junya Kobayashi, Steven M. Yannone, David J. Chen, Aroumougame Asaithamby

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Abstract

WRN, the protein defective in Werner syndrome (WS), is a multifunctional nuclease involved in DNA damage repair, replication, and genome stability maintenance. It was assumed that the nuclease activities of WRN were critical for these functions. Here, we report a nonenzymatic role for WRN in preserving nascent DNA strands following replication stress. We found that lack of WRN led to shortening of nascent DNA strands after replication stress. Furthermore, we discovered that the exonuclease activity of MRE11 was responsible for the shortening of newly replicated DNA in the absence of WRN. Mechanistically, the N-terminal FHA domain of NBS1 recruits WRN to replication-associated DNA double-stranded breaks to stabilize Rad51 and to limit the nuclease activity of its C-terminal binding partner MRE11. Thus, this previously unrecognized nonenzymatic function of WRN in the stabilization of nascent DNA strands sheds light on the molecular reason for the origin of genome instability in WS individuals.

Original languageEnglish (US)
Pages (from-to)1387-1401
Number of pages15
JournalCell Reports
Volume9
Issue number4
DOIs
StatePublished - Nov 20 2014

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Su, F., Mukherjee, S., Yang, Y., Mori, E., Bhattacharya, S., Kobayashi, J., Yannone, S. M., Chen, D. J., & Asaithamby, A. (2014). Nonenzymatic Role for WRN in Preserving Nascent DNA Strands after Replication Stress. Cell Reports, 9(4), 1387-1401. https://doi.org/10.1016/j.celrep.2014.10.025