Nonhematopoietic tumor cells express functional GM-CSF receptors

G. C. Baldwin, J. C. Gasson, S. E. Kaufman, S. G. Quan, R. E. Williams, B. R. Avalos, A. F. Gazdar, D. W. Golde, J. F. DiPersio

Research output: Contribution to journalArticle

165 Citations (Scopus)

Abstract

Human granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulates the colony growth of myeloid progenitors in semisolid media, and enhances the function of mature effector cells, including neutrophils, monocytes, and eosinophils. Small cell carcinoma lines (SCCL) have properties of amine precursor uptake and decarboxylation (APUD) cells and express high levels of enzyme, L-aromatic amino acid decarboxylase. We looked for possible expression of GM-CSF receptors on nonhematopoietic cells and found specific high-affinity binding of human GM-CSF to SCCL and to the SV40-transformed African green monkey kidney cell line, COS. The small cell carcinoma lines responded to GM-CSF with enhanced proliferation, and both small cells and COS cells were found to express authentic 84,000 dalton GM-CSF receptor protein. These findings indicate that nonhematopoietic cells can bind and respond to GM-CSF, suggesting additional biological activities as well as the possibility of tumor responses when GM-CSF is used therapeutically in humans. Since preliminary clinical trials using CSFs as adjunctive treatment in patients with solid tumors are underway, it will be important to consider the possible responsiveness of nonhematopoietic tumor cells to CSFs.

Original languageEnglish (US)
Pages (from-to)1033-1037
Number of pages5
JournalBlood
Volume73
Issue number4
StatePublished - 1989

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Granulocyte-Macrophage Colony-Stimulating Factor Receptors
Granulocyte-Macrophage Colony-Stimulating Factor
Tumors
Cells
Small Cell Carcinoma
Cell Line
Neoplasms
APUD Cells
Aromatic-L-Amino-Acid Decarboxylases
Cercopithecus aethiops
COS Cells
Bioactivity
Eosinophils
Amines
Monocytes
Neutrophils
Clinical Trials
Kidney
Enzymes
Growth

ASJC Scopus subject areas

  • Hematology

Cite this

Baldwin, G. C., Gasson, J. C., Kaufman, S. E., Quan, S. G., Williams, R. E., Avalos, B. R., ... DiPersio, J. F. (1989). Nonhematopoietic tumor cells express functional GM-CSF receptors. Blood, 73(4), 1033-1037.

Nonhematopoietic tumor cells express functional GM-CSF receptors. / Baldwin, G. C.; Gasson, J. C.; Kaufman, S. E.; Quan, S. G.; Williams, R. E.; Avalos, B. R.; Gazdar, A. F.; Golde, D. W.; DiPersio, J. F.

In: Blood, Vol. 73, No. 4, 1989, p. 1033-1037.

Research output: Contribution to journalArticle

Baldwin, GC, Gasson, JC, Kaufman, SE, Quan, SG, Williams, RE, Avalos, BR, Gazdar, AF, Golde, DW & DiPersio, JF 1989, 'Nonhematopoietic tumor cells express functional GM-CSF receptors', Blood, vol. 73, no. 4, pp. 1033-1037.
Baldwin GC, Gasson JC, Kaufman SE, Quan SG, Williams RE, Avalos BR et al. Nonhematopoietic tumor cells express functional GM-CSF receptors. Blood. 1989;73(4):1033-1037.
Baldwin, G. C. ; Gasson, J. C. ; Kaufman, S. E. ; Quan, S. G. ; Williams, R. E. ; Avalos, B. R. ; Gazdar, A. F. ; Golde, D. W. ; DiPersio, J. F. / Nonhematopoietic tumor cells express functional GM-CSF receptors. In: Blood. 1989 ; Vol. 73, No. 4. pp. 1033-1037.
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