Noninvasive evaluation of liver metabolism by 2H and 13C NMR isotopomer analysis of human urine

Shawn C. Burgess, Brian Weis, John G. Jones, Erin Smith, Matthew E. Merritt, David Margolis, A. Dean Sherry, Craig R. Malloy

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Mammalian liver disposes of acetaminophen and other ingested xenobiotics by forming soluble glucuronides that are subsequently removed via renal filtration. When given in combination with the stable isotopes 2H and 13C, the glucuronide of acetaminophen isolated from urine provides a convenient "chemical biopsy" for evaluating intermediary metabolism in the liver. Here, we describe isolation and purification of urinary acetaminophen glucuronide and its conversion to monoacetone glucose (MAG). Subsequent 2H and 13C NMR analysis of MAG from normal volunteers after ingestion of 2H2O and [U-13C3]propionate allowed a noninvasive profiling of hepatic gluconeogenic pathways. The method should find use in metabolic studies of infants and other populations where blood sampling is either limited or problematic.

Original languageEnglish (US)
Pages (from-to)228-234
Number of pages7
JournalAnalytical biochemistry
Volume312
Issue number2
DOIs
StatePublished - Jan 15 2003

Keywords

  • C-isotopomer analysis
  • Citric acid cycle
  • Gluconeogenesis
  • Glycogenolysis
  • H NMR
  • Urine

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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