Nonneuronal expression of Rab3A

Induction during adipogenesis and association with different intracellular membranes than Rab3D

G. Baldini, P. E. Scherer, H. F. Lodish

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Rab3A is a small GTP-binding protein expressed predominantly in brain and neuroendocrine cells, in which it is associated with synaptic and synaptic- like vesicles, respectively. Here we report that adult mouse fat cells and 3T3-L1 adipocytes also express Rab3A mRNA and protein. They do not express synaptophysin, an abundant protein in synaptic vesicles or synaptic-like vesicles. The amount of Rab3A mRNA and protein, like that of the highly homologous isoform Rab3D, increases severalfold during differentiation of 3T3-L1 fibroblasts into mature adipocytes. In fat cells, most Rab3D and Rab3A protein is bound to membrane, irrespective of insulin addition. Rab3A and Rab3D are localized in different subcellular compartments, since about half of the Rab3A, but none of the Rab3D, is associated with a low-density organelle(s). Rab3D and Rab3A may be involved in different pathways of regulated exocytosis in adipocytes. Moreover, in adipocytes Rab3A may define an exocytic organelle that is different from synaptic vesicles or synaptic- like microvesicles found in neuronal and endocrine cells.

Original languageEnglish (US)
Pages (from-to)4284-4288
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number10
StatePublished - 1995

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Adipogenesis
Intracellular Membranes
Adipocytes
rab3A GTP-Binding Protein
Synaptic Vesicles
Organelles
Neuroendocrine Cells
Messenger RNA
Synaptophysin
Endocrine Cells
Exocytosis
GTP-Binding Proteins
Protein Isoforms
Fibroblasts
Insulin
Membranes
Brain
Proteins

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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abstract = "Rab3A is a small GTP-binding protein expressed predominantly in brain and neuroendocrine cells, in which it is associated with synaptic and synaptic- like vesicles, respectively. Here we report that adult mouse fat cells and 3T3-L1 adipocytes also express Rab3A mRNA and protein. They do not express synaptophysin, an abundant protein in synaptic vesicles or synaptic-like vesicles. The amount of Rab3A mRNA and protein, like that of the highly homologous isoform Rab3D, increases severalfold during differentiation of 3T3-L1 fibroblasts into mature adipocytes. In fat cells, most Rab3D and Rab3A protein is bound to membrane, irrespective of insulin addition. Rab3A and Rab3D are localized in different subcellular compartments, since about half of the Rab3A, but none of the Rab3D, is associated with a low-density organelle(s). Rab3D and Rab3A may be involved in different pathways of regulated exocytosis in adipocytes. Moreover, in adipocytes Rab3A may define an exocytic organelle that is different from synaptic vesicles or synaptic- like microvesicles found in neuronal and endocrine cells.",
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