TY - JOUR
T1 - Normal human colonic subepithelial myofibroblasts enhance epithelial migration (restitution) via TGF-β3
AU - McKaig, Brian C.
AU - Makh, Shavcharn S.
AU - Hawkey, Christopher J.
AU - Podolsky, Daniel K.
AU - Mahida, Yashwant R.
PY - 1999/5
Y1 - 1999/5
N2 - After injury and loss of epithelial cells, intestinal barrier function is reestablished by migration of viable epithelial cells from the wound edge (restitution). Myofibroblasts are located close to the basal surface of epithelial cells. This study aimed to investigate the role of human colonic subepithelial myofibroblasts in epithelial restitution. Primary cultures of subepithelial myofibroblasts were established. Monolayers of the epithelial cell lines IEC-6 and T84 were 'wounded' in a standard manner to create an in vitro model of restitution. Migration of epithelial cells across the wound edge was assessed following culture in myofibroblast-conditioned medium. Myofibroblast expression of transforming growth factor (TGF)-β isoforms was examined using RT-PCR, and TGF-β isoform bioactivity was assessed using Mv 1 Lu bioassay. Myofibroblast-conditioned medium, via a TGF-β-dependent pathway, significantly enhanced migration of epithelial cells across the wound edge and significantly inhibited cell proliferation in wounded monolayers. Messenger RNA for TGF-β1, -β2, and -β3 was detected in the myofibroblasts, and Mv 1 Lu bioassay showed the presence of predominantly bioactive TGF-β3. This study shows that human colonic subepithelial myofibroblasts secrete predominantly bioactive TGF-β3 and enhance restitution in wounded epithelial monolayers via a TGF-β-dependent pathway.
AB - After injury and loss of epithelial cells, intestinal barrier function is reestablished by migration of viable epithelial cells from the wound edge (restitution). Myofibroblasts are located close to the basal surface of epithelial cells. This study aimed to investigate the role of human colonic subepithelial myofibroblasts in epithelial restitution. Primary cultures of subepithelial myofibroblasts were established. Monolayers of the epithelial cell lines IEC-6 and T84 were 'wounded' in a standard manner to create an in vitro model of restitution. Migration of epithelial cells across the wound edge was assessed following culture in myofibroblast-conditioned medium. Myofibroblast expression of transforming growth factor (TGF)-β isoforms was examined using RT-PCR, and TGF-β isoform bioactivity was assessed using Mv 1 Lu bioassay. Myofibroblast-conditioned medium, via a TGF-β-dependent pathway, significantly enhanced migration of epithelial cells across the wound edge and significantly inhibited cell proliferation in wounded monolayers. Messenger RNA for TGF-β1, -β2, and -β3 was detected in the myofibroblasts, and Mv 1 Lu bioassay showed the presence of predominantly bioactive TGF-β3. This study shows that human colonic subepithelial myofibroblasts secrete predominantly bioactive TGF-β3 and enhance restitution in wounded epithelial monolayers via a TGF-β-dependent pathway.
KW - Transforming growth factor-β
KW - Wound repair
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U2 - 10.1152/ajpgi.1999.276.5.g1087
DO - 10.1152/ajpgi.1999.276.5.g1087
M3 - Article
C2 - 10329998
AN - SCOPUS:0033049460
SN - 0193-1857
VL - 276
SP - G1087-G1093
JO - American Journal of Physiology - Gastrointestinal and Liver Physiology
JF - American Journal of Physiology - Gastrointestinal and Liver Physiology
IS - 5 39-5
ER -