NOTPME: A 31P NMR probe for measurementof divalent cations in biological systems

Ravichandran Ramasamy, Istvan Lazar, Erno Brucher, A. Dean Sherry, Craig R. Malloy

Research output: Contribution to journalArticle

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Abstract

1, 4, 7-Triazacyclononame-N, N′,N′-tris(methylenephosphonate monoethylester) (NOTPME) has been synthesized, characterized and analyzed for use as a 31P NMR indicator of intracellular Mg2+ and Zn2+ ions. The 31P NMR spectrum of this chelate in the presence of metal ions shows characteristics resonances for the free chelate, Mg(NOTPME)-, Zn(NOTPME)- and Ca(NOTPME)-. The K??? values indicate that this chelate has a 10-fold higher affinity for Mg2+ than for Ca2+ at physiological pH values. In the presence of Mg2+, NOTPME is readily loaded into red blood cells. A 31P NMR spectrum of red cells taken after several washing shows resonances characteristics of entrapped NOTPME and the Mg(NOTPME)- complex, the relative areas of which report an intracellular free Mg2+ concentration of 0.32 mM. The 31P chemical shifts of the free chelate and its metal complexes are far downfield from the typical phosphorus-containing metabolites observed in biological systems, thus making it possible to monitor intracellular cation concentration and cell energetics simultaneously.

Original languageEnglish (US)
Pages (from-to)121-124
Number of pages4
JournalFEBS Letters
Volume280
Issue number1
DOIs
StatePublished - Mar 11 1991

Fingerprint

Divalent Cations
Biological systems
Nuclear magnetic resonance
Cells
Ions
Coordination Complexes
Chemical shift
Metabolites
1,4,7-triazacyclononane-N,N',N''-tris(methylenephosphonate monoethyl ester)
Washing
Phosphorus
Metal ions
Cations
Blood
Erythrocytes
Metals

Keywords

  • P NMR indicator
  • Magnesium chelator

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

NOTPME : A 31P NMR probe for measurementof divalent cations in biological systems. / Ramasamy, Ravichandran; Lazar, Istvan; Brucher, Erno; Sherry, A. Dean; Malloy, Craig R.

In: FEBS Letters, Vol. 280, No. 1, 11.03.1991, p. 121-124.

Research output: Contribution to journalArticle

Ramasamy, Ravichandran ; Lazar, Istvan ; Brucher, Erno ; Sherry, A. Dean ; Malloy, Craig R. / NOTPME : A 31P NMR probe for measurementof divalent cations in biological systems. In: FEBS Letters. 1991 ; Vol. 280, No. 1. pp. 121-124.
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