Novel antibodies reacting with two neighboring gangliosides are induced in rabbits immunized with bovine brain gangliosides

Ana L. Moyano, Romina Comín, Aldo Alejandro Vilcaes, Samanta C. Funes, Germán A. Roth, Fernando J. Irazoqui, Gustavo A. Nores

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Immunization of rabbits with bovine brain gangliosides induced an experimental neuropathy, with clinical signs resembling Guillain-Barré syndrome. All the immunized animals developed immunoglobulin G immunoreactivity to GM1 ganglioside. In a few (4 of 27) animals, an additional anti-ganglioside antibody population showing an unusual binding behavior was detected. Enzyme-linked immunosorbent assay and thin-layer chromatography immunostaining analyses showed that the binding of these unusual antibodies required the presence of two co-localized gangliosides. Maximal interaction was observed to a mixture of GM1 and GD1b, but the antibodies also showed density-dependent binding to GD1b. The antibodies were purified by affinity chromatography and displayed the ability to target antigens in biological membranes (rat synaptosomes).

Original languageEnglish (US)
Pages (from-to)1768-1774
Number of pages7
JournalGlycobiology
Volume22
Issue number12
DOIs
StatePublished - Dec 1 2012
Externally publishedYes

Fingerprint

Gangliosides
Brain
Rabbits
Antibodies
G(M1) Ganglioside
Animals
Aptitude
Synaptosomes
Thin Layer Chromatography
Immunization
Biological membranes
Affinity Chromatography
Affinity chromatography
Thin layer chromatography
Immunosorbents
Anti-Idiotypic Antibodies
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Antigens
Rats

Keywords

  • density-dependent binding
  • experimental neuropathy
  • ganglioside complex
  • gangliosides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Novel antibodies reacting with two neighboring gangliosides are induced in rabbits immunized with bovine brain gangliosides. / Moyano, Ana L.; Comín, Romina; Vilcaes, Aldo Alejandro; Funes, Samanta C.; Roth, Germán A.; Irazoqui, Fernando J.; Nores, Gustavo A.

In: Glycobiology, Vol. 22, No. 12, 01.12.2012, p. 1768-1774.

Research output: Contribution to journalArticle

Moyano, Ana L. ; Comín, Romina ; Vilcaes, Aldo Alejandro ; Funes, Samanta C. ; Roth, Germán A. ; Irazoqui, Fernando J. ; Nores, Gustavo A. / Novel antibodies reacting with two neighboring gangliosides are induced in rabbits immunized with bovine brain gangliosides. In: Glycobiology. 2012 ; Vol. 22, No. 12. pp. 1768-1774.
@article{104acdf1a58a4f0e9317b092ff3e3b54,
title = "Novel antibodies reacting with two neighboring gangliosides are induced in rabbits immunized with bovine brain gangliosides",
abstract = "Immunization of rabbits with bovine brain gangliosides induced an experimental neuropathy, with clinical signs resembling Guillain-Barr{\'e} syndrome. All the immunized animals developed immunoglobulin G immunoreactivity to GM1 ganglioside. In a few (4 of 27) animals, an additional anti-ganglioside antibody population showing an unusual binding behavior was detected. Enzyme-linked immunosorbent assay and thin-layer chromatography immunostaining analyses showed that the binding of these unusual antibodies required the presence of two co-localized gangliosides. Maximal interaction was observed to a mixture of GM1 and GD1b, but the antibodies also showed density-dependent binding to GD1b. The antibodies were purified by affinity chromatography and displayed the ability to target antigens in biological membranes (rat synaptosomes).",
keywords = "density-dependent binding, experimental neuropathy, ganglioside complex, gangliosides",
author = "Moyano, {Ana L.} and Romina Com{\'i}n and Vilcaes, {Aldo Alejandro} and Funes, {Samanta C.} and Roth, {Germ{\'a}n A.} and Irazoqui, {Fernando J.} and Nores, {Gustavo A.}",
year = "2012",
month = "12",
day = "1",
doi = "10.1093/glycob/cws117",
language = "English (US)",
volume = "22",
pages = "1768--1774",
journal = "Glycobiology",
issn = "0959-6658",
publisher = "Oxford University Press",
number = "12",

}

TY - JOUR

T1 - Novel antibodies reacting with two neighboring gangliosides are induced in rabbits immunized with bovine brain gangliosides

AU - Moyano, Ana L.

AU - Comín, Romina

AU - Vilcaes, Aldo Alejandro

AU - Funes, Samanta C.

AU - Roth, Germán A.

AU - Irazoqui, Fernando J.

AU - Nores, Gustavo A.

PY - 2012/12/1

Y1 - 2012/12/1

N2 - Immunization of rabbits with bovine brain gangliosides induced an experimental neuropathy, with clinical signs resembling Guillain-Barré syndrome. All the immunized animals developed immunoglobulin G immunoreactivity to GM1 ganglioside. In a few (4 of 27) animals, an additional anti-ganglioside antibody population showing an unusual binding behavior was detected. Enzyme-linked immunosorbent assay and thin-layer chromatography immunostaining analyses showed that the binding of these unusual antibodies required the presence of two co-localized gangliosides. Maximal interaction was observed to a mixture of GM1 and GD1b, but the antibodies also showed density-dependent binding to GD1b. The antibodies were purified by affinity chromatography and displayed the ability to target antigens in biological membranes (rat synaptosomes).

AB - Immunization of rabbits with bovine brain gangliosides induced an experimental neuropathy, with clinical signs resembling Guillain-Barré syndrome. All the immunized animals developed immunoglobulin G immunoreactivity to GM1 ganglioside. In a few (4 of 27) animals, an additional anti-ganglioside antibody population showing an unusual binding behavior was detected. Enzyme-linked immunosorbent assay and thin-layer chromatography immunostaining analyses showed that the binding of these unusual antibodies required the presence of two co-localized gangliosides. Maximal interaction was observed to a mixture of GM1 and GD1b, but the antibodies also showed density-dependent binding to GD1b. The antibodies were purified by affinity chromatography and displayed the ability to target antigens in biological membranes (rat synaptosomes).

KW - density-dependent binding

KW - experimental neuropathy

KW - ganglioside complex

KW - gangliosides

UR - http://www.scopus.com/inward/record.url?scp=84868597665&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84868597665&partnerID=8YFLogxK

U2 - 10.1093/glycob/cws117

DO - 10.1093/glycob/cws117

M3 - Article

C2 - 22843673

AN - SCOPUS:84868597665

VL - 22

SP - 1768

EP - 1774

JO - Glycobiology

JF - Glycobiology

SN - 0959-6658

IS - 12

ER -