Novel approaches to bacterial infection therapy by interfering with cell-to-cell signaling

David A. Rasko, Vanessa Sperandio

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The identification of cell-to-cell signaling inhibitors among bacteria is a novel mechanism used to control the virulence of pathogens. Signaling inhibitors have a significant potential to be used as therapeutics in that they will exert less biological pressure to develop resistance than classic antibiotics, which attempt to eradicate or significantly inhibit bacterial growth. Decreasing the virulence of pathogens by inhibiting signaling provides a mechanism by which the bacteria will be "misled" into not activating virulence factors. If virulence factors are not activated, these pathogens will either pass through the host without incidence or be controlled by the host's immune system. In this unit, we discuss the general principles for the design and implementation of a highthroughput screen to identify inhibitors of bacterial cell-to-cell communication. We also provide a detailed protocol using a specific example of signaling inhibitor identification using enterohemorrhagic Escherichia coli as a model system.

Original languageEnglish (US)
JournalCurrent Protocols in Microbiology
Issue numberSUPPL. 12
DOIs
StatePublished - 2009

Fingerprint

Bacterial Infections
Virulence Factors
Virulence
Enterohemorrhagic Escherichia coli
Bacteria
Microbial Drug Resistance
Cell Communication
Immune System
Therapeutics
Pressure
Incidence
Growth

Keywords

  • Autoinducer
  • Cell-to-cell signaling
  • Escherichia coli
  • Inhibitor
  • Virulence

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology

Cite this

Novel approaches to bacterial infection therapy by interfering with cell-to-cell signaling. / Rasko, David A.; Sperandio, Vanessa.

In: Current Protocols in Microbiology, No. SUPPL. 12, 2009.

Research output: Contribution to journalArticle

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