Novel genomic cDNA hybrids produce effective RNA interference in adult Drosophila

Savitha Kalidas, Dean P. Smith

Research output: Contribution to journalArticlepeer-review

181 Scopus citations

Abstract

Drosophila melanogaster has been a premier genetic model system for nearly 100 years, yet lacks a simple method to disrupt gene expression. Here, we show genomic cDNA fusions predicted to form double-stranded RNA (dsRNA) following splicing, effectively silencing expression of target genes in adult transgenic animals. We targeted three Drosophila genes: lush, white, and dGqα. In each case, target gene expression is dramatically reduced, and the white RNAi phenotype is indistinguishable from a deletion mutant. This technique efficiently targets genes expressed in neurons, a tissue refractory to RNAi in C. elegans. These results demonstrate a simple strategy to knock out gene function in specific cells in living adult Drosophila that can be applied to define the biological function of hundreds of orphan genes and open reading frames.

Original languageEnglish (US)
Pages (from-to)177-184
Number of pages8
JournalNeuron
Volume33
Issue number2
DOIs
StatePublished - Jan 17 2002

ASJC Scopus subject areas

  • Neuroscience(all)

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