Novel heme-based oxygen sensor with a revealing evolutionary history

Oleg V. Moskvin, Samuel Kaplan, Marie Alda Gilles-Gonzalez, Mark Gomelsky

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

To monitor fluctuations in oxygen concentration, cells use sensory proteins often containing heme cofactors. Here, we identify a new class of heme-binding oxygen sensors, reveal their unusual phylogenetic origin, and propose a sensing mode of a member of this class. We show that heme is bound noncovalently to the central region of AppA, an oxygen and light sensor from Rhodobacter sphaeroides. The addition of oxygen to ferrous AppA discoordinated the heme, and subsequent oxygen removal fully restored the heme coordination. In vitro, the extent of heme discoordination increased gradually with the rise in oxygen levels over a broad concentration range. This response correlated well with the gradual decrease in transcription of photosynthesis genes regulated by AppA and its partner repressor PpsR. We conclude that the AppA-PpsR regulatory system functions as an oxygen-dependent transcriptional rheostat. We identified a new domain embedded in the central region of AppA and designated it SCHIC for sensor containing heme instead of cobalamin. A phylogenetic analysis revealed that SCHIC domain proteins form a distinct cluster within a superfamily that includes vitamin B12-binding proteins and other proteins that may bind other kinds of tetrapyrroles.

Original languageEnglish (US)
Pages (from-to)28740-28748
Number of pages9
JournalJournal of Biological Chemistry
Volume282
Issue number39
DOIs
StatePublished - Sep 28 2007

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Oxygen sensors
Heme
History
Oxygen
Tetrapyrroles
Transcobalamins
Rhodobacter sphaeroides
Proteins
Photosynthesis
Sensors
Transcription
Vitamin B 12
Genes
diadenosine pyrophosphate
Light

ASJC Scopus subject areas

  • Biochemistry

Cite this

Novel heme-based oxygen sensor with a revealing evolutionary history. / Moskvin, Oleg V.; Kaplan, Samuel; Gilles-Gonzalez, Marie Alda; Gomelsky, Mark.

In: Journal of Biological Chemistry, Vol. 282, No. 39, 28.09.2007, p. 28740-28748.

Research output: Contribution to journalArticle

Moskvin, Oleg V. ; Kaplan, Samuel ; Gilles-Gonzalez, Marie Alda ; Gomelsky, Mark. / Novel heme-based oxygen sensor with a revealing evolutionary history. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 39. pp. 28740-28748.
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