Novel metal-binding proteins by design

Michael Klemba, Kevin H. Gardner, Stephen Marino, Neil D. Clarke, Lynne Regan

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

We describe the successful design of a tetrahedral His3Cys Zn(II)-binding site in a small protein of known structure: the B1 domain of Streptococcal protein G. The B1 variants containing the novel metal-binding site were characterized using a combination of optical absorption, circular dichroism and NMR spectroscopies. The results indicate that the designed proteins bind Zn(II) with high affinity and tetrahedral coordination geometry, and that the overall secondary and tertiary structure of the B1 domain is maintained.

Original languageEnglish (US)
Pages (from-to)368-373
Number of pages6
JournalNature Structural Biology
Volume2
Issue number5
StatePublished - May 1995

Fingerprint

Carrier Proteins
Metals
Binding Sites
Circular dichroism spectroscopy
Circular Dichroism
Light absorption
Nuclear magnetic resonance spectroscopy
Proteins
Magnetic Resonance Spectroscopy
Geometry
Streptococcus IgG Fc-binding protein

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology

Cite this

Klemba, M., Gardner, K. H., Marino, S., Clarke, N. D., & Regan, L. (1995). Novel metal-binding proteins by design. Nature Structural Biology, 2(5), 368-373.

Novel metal-binding proteins by design. / Klemba, Michael; Gardner, Kevin H.; Marino, Stephen; Clarke, Neil D.; Regan, Lynne.

In: Nature Structural Biology, Vol. 2, No. 5, 05.1995, p. 368-373.

Research output: Contribution to journalArticle

Klemba, M, Gardner, KH, Marino, S, Clarke, ND & Regan, L 1995, 'Novel metal-binding proteins by design', Nature Structural Biology, vol. 2, no. 5, pp. 368-373.
Klemba M, Gardner KH, Marino S, Clarke ND, Regan L. Novel metal-binding proteins by design. Nature Structural Biology. 1995 May;2(5):368-373.
Klemba, Michael ; Gardner, Kevin H. ; Marino, Stephen ; Clarke, Neil D. ; Regan, Lynne. / Novel metal-binding proteins by design. In: Nature Structural Biology. 1995 ; Vol. 2, No. 5. pp. 368-373.
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