Novel metal-binding proteins by design

Michael Klemba, Kevin H. Gardner, Stephen Marino, Neil D. Clarke, Lynne Regan

Research output: Contribution to journalArticlepeer-review

94 Scopus citations


We describe the successful design of a tetrahedral His3Cys Zn (II)-binding site in a small protein of known structure: the B1 domain of Streptococcal protein G. The B1 variants containing the novel metal-binding site were characterized using a combination of optical absorption, circular dichroism and NMR spectroscopies. The results indicate that the designed proteins bind Zn (II) with high affinity and tetrahedral coordination geometry, and that the overall secondary and tertiary structure of the B1 domain is maintained.

Original languageEnglish (US)
Pages (from-to)368-373
Number of pages6
JournalNature Structural Biology
Issue number5
StatePublished - May 1995

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Genetics


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