TY - JOUR
T1 - Novel receptor-targeted fluorescent contrast agents for in vivo tumor imaging
AU - Achilefu, Samuel
AU - Dorshow, Richard B.
AU - Bugaj, Joseph E.
AU - Rajagopalan, Raghavan
PY - 2000
Y1 - 2000
N2 - RATIONALE AND OBJECTIVES. To evaluate the efficacy of a novel tumor receptor-specific small-peptide-near-infrared dye conjugate for tumor detection by optical imaging. METHODS. A novel, near-infrared dye-peptide conjugate was synthesized and evaluated for tumor-targeting efficacy in a well-characterized rat tumor model (CA20948) known to express receptors for the chosen peptide. A simple continuous-wave optical imaging system, consisting of a near-infrared laser diode, a cooled CCD camera, and an interference filter, was used in this study. RESULTS. Tumor retention of two non-tumor-specific dyes, indocyanine green and its derivatized analogue, bis-propanoic acid cyanine dye (cypate), was negligible. In contrast, the receptor-specific peptide-cypate conjugate (cytate) was retained in the CA20948 tumor, with an excellent tumor-to-normal-tissue ratio in the six rats examined. CONCLUSIONS. Optical detection of tumors with a receptor-targeted fluorescent contrast agent has been demonstrated. This result represents a new direction in cancer diagnosis and patient management.
AB - RATIONALE AND OBJECTIVES. To evaluate the efficacy of a novel tumor receptor-specific small-peptide-near-infrared dye conjugate for tumor detection by optical imaging. METHODS. A novel, near-infrared dye-peptide conjugate was synthesized and evaluated for tumor-targeting efficacy in a well-characterized rat tumor model (CA20948) known to express receptors for the chosen peptide. A simple continuous-wave optical imaging system, consisting of a near-infrared laser diode, a cooled CCD camera, and an interference filter, was used in this study. RESULTS. Tumor retention of two non-tumor-specific dyes, indocyanine green and its derivatized analogue, bis-propanoic acid cyanine dye (cypate), was negligible. In contrast, the receptor-specific peptide-cypate conjugate (cytate) was retained in the CA20948 tumor, with an excellent tumor-to-normal-tissue ratio in the six rats examined. CONCLUSIONS. Optical detection of tumors with a receptor-targeted fluorescent contrast agent has been demonstrated. This result represents a new direction in cancer diagnosis and patient management.
KW - Contrast agents
KW - Dye-peptide conjugates
KW - Optical imaging
KW - Tumor targeting
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U2 - 10.1097/00004424-200008000-00004
DO - 10.1097/00004424-200008000-00004
M3 - Article
C2 - 10946975
AN - SCOPUS:0033853378
SN - 0020-9996
VL - 35
SP - 479
EP - 485
JO - Investigative Radiology
JF - Investigative Radiology
IS - 8
ER -