Novel role of estrogen receptors in vascular endothelium

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Estrogen has a variety of effects on the vascular wall including rapid vasodilation due to the stimulation of endothelial nitric oxide synthase (eNOS). Studies in cultured endothelium indicate that the hormone cause acute, direct activation of eNOS that is unaffected by actinomycin D but fully inhibited by estrogen receptor (ER) antagonism. Overexpression of ERα leads to marked enhancement of the acute response to estrogen, and this process is blocked by ER antagonism and requires the ERα hormone binding domain. The acute response of eNOS to estrogen can also be reconstituted in COS-7 cells cotransfected with ERα and eNOS, but not by transfection with eNOS alone. The inhibition of calcium influx, or tyrosine kinases or mitogen- activated protein (MAP) kinase prevents eNOS stimulation by estrogen, and estrogen causes rapid ER-dependent activation of MAP kinase. Thus, the acute effect of estrogen on eNOS is mediated by ERα functioning in a novel, nongenomic manner to activate the enzyme via calcium-dependent, MAP kinase- dependent mechanisms. Copyright (C) 2000 by W.B. Saunders Company.

Original languageEnglish (US)
Pages (from-to)70-74
Number of pages5
JournalSeminars in Perinatology
Volume24
Issue number1
StatePublished - 2000

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Nitric Oxide Synthase Type III
Vascular Endothelium
Estrogen Receptors
Estrogens
Mitogen-Activated Protein Kinases
Hormones
Calcium
COS Cells
Dactinomycin
Vasodilation
Protein-Tyrosine Kinases
Endothelium
Transfection
Blood Vessels
Enzymes

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Novel role of estrogen receptors in vascular endothelium. / Shaul, Philip W.

In: Seminars in Perinatology, Vol. 24, No. 1, 2000, p. 70-74.

Research output: Contribution to journalArticle

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