Novel sequence feature variant type analysis of the HLA genetic association in systemic sclerosis

David R. Karp, Nishanth Marthandan, Steven G E Marsh, Chul Ahn, Frank C. Arnett, David S. DeLuca, Alexander D. Diehl, Raymond Dunivin, Karen Eilbeck, Michael Feolo, Paula A. Guidry, Wolfgang Helmberg, Suzanna Lewis, Maureen D. Mayes, Chris Mungall, Darren A. Natale, Bjoern Peters, Effie Petersdorf, John D. Reveille, Barry SmithGlenys Thomson, Matthew J. Waller, Richard H. Scheuermann

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We describe a novel approach to genetic association analyses with proteins sub-divided into biologically relevant smaller sequence features (SFs), and their variant types (VTs). SFVT analyses are particularly informative for study of highly polymorphic proteins such as the human leukocyte antigen (HLA), given the nature of its genetic variation: the high level of polymorphism, the pattern of amino acid variability, and that most HLA variation occurs at functionally important sites, as well as its known role in organ transplant rejection, autoimmune disease development and response to infection. Further, combinations of variable amino acid sites shared by several HLA alleles (shared epitopes) are most likely better descriptors of the actual causative genetic variants. In a cohort of systemic sclerosis patients/controls, SFVT analysis shows that a combination of SFs implicating specific amino acid residues in peptide binding pockets 4 and 7 of HLA-DRB1 explains much of the molecular determinant of risk.

Original languageEnglish (US)
Article numberddp521
Pages (from-to)707-719
Number of pages13
JournalHuman Molecular Genetics
Volume19
Issue number4
DOIs
StatePublished - Nov 18 2009

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Systemic Scleroderma
HLA Antigens
Amino Acids
Antigenic Variation
Graft Rejection
Autoimmunity
Autoimmune Diseases
Epitopes
Proteins
Alleles
Transplants
Peptides
Infection

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Molecular Biology

Cite this

Karp, D. R., Marthandan, N., Marsh, S. G. E., Ahn, C., Arnett, F. C., DeLuca, D. S., ... Scheuermann, R. H. (2009). Novel sequence feature variant type analysis of the HLA genetic association in systemic sclerosis. Human Molecular Genetics, 19(4), 707-719. [ddp521]. https://doi.org/10.1093/hmg/ddp521

Novel sequence feature variant type analysis of the HLA genetic association in systemic sclerosis. / Karp, David R.; Marthandan, Nishanth; Marsh, Steven G E; Ahn, Chul; Arnett, Frank C.; DeLuca, David S.; Diehl, Alexander D.; Dunivin, Raymond; Eilbeck, Karen; Feolo, Michael; Guidry, Paula A.; Helmberg, Wolfgang; Lewis, Suzanna; Mayes, Maureen D.; Mungall, Chris; Natale, Darren A.; Peters, Bjoern; Petersdorf, Effie; Reveille, John D.; Smith, Barry; Thomson, Glenys; Waller, Matthew J.; Scheuermann, Richard H.

In: Human Molecular Genetics, Vol. 19, No. 4, ddp521, 18.11.2009, p. 707-719.

Research output: Contribution to journalArticle

Karp, DR, Marthandan, N, Marsh, SGE, Ahn, C, Arnett, FC, DeLuca, DS, Diehl, AD, Dunivin, R, Eilbeck, K, Feolo, M, Guidry, PA, Helmberg, W, Lewis, S, Mayes, MD, Mungall, C, Natale, DA, Peters, B, Petersdorf, E, Reveille, JD, Smith, B, Thomson, G, Waller, MJ & Scheuermann, RH 2009, 'Novel sequence feature variant type analysis of the HLA genetic association in systemic sclerosis', Human Molecular Genetics, vol. 19, no. 4, ddp521, pp. 707-719. https://doi.org/10.1093/hmg/ddp521
Karp, David R. ; Marthandan, Nishanth ; Marsh, Steven G E ; Ahn, Chul ; Arnett, Frank C. ; DeLuca, David S. ; Diehl, Alexander D. ; Dunivin, Raymond ; Eilbeck, Karen ; Feolo, Michael ; Guidry, Paula A. ; Helmberg, Wolfgang ; Lewis, Suzanna ; Mayes, Maureen D. ; Mungall, Chris ; Natale, Darren A. ; Peters, Bjoern ; Petersdorf, Effie ; Reveille, John D. ; Smith, Barry ; Thomson, Glenys ; Waller, Matthew J. ; Scheuermann, Richard H. / Novel sequence feature variant type analysis of the HLA genetic association in systemic sclerosis. In: Human Molecular Genetics. 2009 ; Vol. 19, No. 4. pp. 707-719.
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