Abstract
Cellular immunity plays an important role in controlling HIV-1 replication. One of the major challenges in developing an HIV-1 DNA vaccine is to generate broader and more potent cellular responses. In this study, we constructed three novel constructs expressing SIVmac antigens Env, Pol and Gag, respectively, with the goal of increasing anti-SIV cellular immunity. The results demonstrate that these constructs can induce strong cellular immune responses in a murine model. Moreover, when applied to cynomolgus macaques, these constructs are not only able to elicit robust IFN-γ effector responses, but also induce SIV antigen-specific CD8+ T cells that have high proliferative capacity. These data suggest that such DNA immunogens deserve further examination for their potential to control viral replication.
Original language | English (US) |
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Pages (from-to) | 3260-3266 |
Number of pages | 7 |
Journal | Vaccine |
Volume | 27 |
Issue number | 25-26 |
DOIs | |
State | Published - May 26 2009 |
Externally published | Yes |
Keywords
- Cellular immune responses
- Consensus
- SIV DNA vaccine
ASJC Scopus subject areas
- Molecular Medicine
- General Immunology and Microbiology
- General Veterinary
- Public Health, Environmental and Occupational Health
- Infectious Diseases