Nuclear control of cellular aging demonstrated by hybridization of anucleate and whole cultured normal human fibroblasts

W. E. Wright, L. Hayflick

Research output: Contribution to journalArticle

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Abstract

Cytoplasmic hybridization was achieved by fusing anucleate cytoplasms to iodoacetate-treated, whole, normal human fibroblasts. The enzymes in the untreated anucleate cytoplasms replaced inactivated enzymes in the treated whole cells and permitted survival of the hybrids. The population doubling potential of cytoplasmic hybrids made between young and old cells was compared with those made between young/young or old/old controls. The results suggest that nuclear rather than cytoplasmic factors are involved in the control of in vitro cellular senescence.

Original languageEnglish (US)
Pages (from-to)113-121
Number of pages9
JournalExperimental Cell Research
Volume96
Issue number1
DOIs
StatePublished - Nov 1975

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ASJC Scopus subject areas

  • Cell Biology

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