Nuclear control of cellular aging demonstrated by hybridization of anucleate and whole cultured normal human fibroblasts

W. E. Wright; L. Hayflick

doi:10.1016/S0014-4827(75)80043-7

Nuclear control of cellular aging demonstrated by hybridization of anucleate and whole cultured normal human fibroblasts

W. E. Wright, L. Hayflick

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Abstract

Cytoplasmic hybridization was achieved by fusing anucleate cytoplasms to iodoacetate-treated, whole, normal human fibroblasts. The enzymes in the untreated anucleate cytoplasms replaced inactivated enzymes in the treated whole cells and permitted survival of the hybrids. The population doubling potential of cytoplasmic hybrids made between young and old cells was compared with those made between young/young or old/old controls. The results suggest that nuclear rather than cytoplasmic factors are involved in the control of in vitro cellular senescence.

Original language	English (US)
Pages (from-to)	113-121
Number of pages	9
Journal	Experimental Cell Research
Volume	96
Issue number	1
DOIs	https://doi.org/10.1016/S0014-4827(75)80043-7
State	Published - Nov 1975

ASJC Scopus subject areas

Cell Biology

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10.1016/S0014-4827(75)80043-7

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title = "Nuclear control of cellular aging demonstrated by hybridization of anucleate and whole cultured normal human fibroblasts",

abstract = "Cytoplasmic hybridization was achieved by fusing anucleate cytoplasms to iodoacetate-treated, whole, normal human fibroblasts. The enzymes in the untreated anucleate cytoplasms replaced inactivated enzymes in the treated whole cells and permitted survival of the hybrids. The population doubling potential of cytoplasmic hybrids made between young and old cells was compared with those made between young/young or old/old controls. The results suggest that nuclear rather than cytoplasmic factors are involved in the control of in vitro cellular senescence.",

author = "Wright, {W. E.} and L. Hayflick",

note = "Funding Information: This study was supported in part by research grant HD 04004 from the National Institute of Child Health and Human Development and by Medical Scientists Training Grant no. GM 1922 from the National Institute of General Medical Sciences, NIH, Bethesda, Maryland, USA.",

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AU - Hayflick, L.

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PY - 1975/11

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N2 - Cytoplasmic hybridization was achieved by fusing anucleate cytoplasms to iodoacetate-treated, whole, normal human fibroblasts. The enzymes in the untreated anucleate cytoplasms replaced inactivated enzymes in the treated whole cells and permitted survival of the hybrids. The population doubling potential of cytoplasmic hybrids made between young and old cells was compared with those made between young/young or old/old controls. The results suggest that nuclear rather than cytoplasmic factors are involved in the control of in vitro cellular senescence.

AB - Cytoplasmic hybridization was achieved by fusing anucleate cytoplasms to iodoacetate-treated, whole, normal human fibroblasts. The enzymes in the untreated anucleate cytoplasms replaced inactivated enzymes in the treated whole cells and permitted survival of the hybrids. The population doubling potential of cytoplasmic hybrids made between young and old cells was compared with those made between young/young or old/old controls. The results suggest that nuclear rather than cytoplasmic factors are involved in the control of in vitro cellular senescence.

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Nuclear control of cellular aging demonstrated by hybridization of anucleate and whole cultured normal human fibroblasts

Abstract

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