Cytoplasmic hybridization was achieved by fusing anucleate cytoplasms to iodoacetate-treated, whole, normal human fibroblasts. The enzymes in the untreated anucleate cytoplasms replaced inactivated enzymes in the treated whole cells and permitted survival of the hybrids. The population doubling potential of cytoplasmic hybrids made between young and old cells was compared with those made between young/young or old/old controls. The results suggest that nuclear rather than cytoplasmic factors are involved in the control of in vitro cellular senescence.
ASJC Scopus subject areas
- Cell Biology