Nuclear foci assays in live cells

Eiichiro Mori, Asaithamby Aroumougame

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

DNA double strand breaks (DSBs) are a serious threat to genome stability and cell viability. Accurate detection of DSBs is critical for the basic understanding of cellular response to ionizing radiation. Recruitment and retention of DNA repair and response proteins at DSBs can be conveniently visualized by fluorescence imaging (often called ionizing radiation-induced foci) both in live and fixed cells. In this chapter, we describe a live cell imaging methodology that directly monitors induction and repair of single DSB, recruitment kinetics of DSB repair/sensor factors to DSB sites, and dynamic interaction of DSB repair/sensor proteins with DSBs at single-cell level. Additionally, the methodology described in this chapter can be readily adapted to other DSBs repair/sensor factors and cell types.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages75-85
Number of pages11
DOIs
Publication statusPublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1984
ISSN (Print)1064-3745

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Keywords

  • 53BP1
  • DNA double strand breaks
  • FRAP
  • Live cell imaging
  • Nuclear foci

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Mori, E., & Aroumougame, A. (2019). Nuclear foci assays in live cells. In Methods in Molecular Biology (pp. 75-85). (Methods in Molecular Biology; Vol. 1984). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9432-8_9