Nuclei isolation from surgically resected human hippocampus

Fatma Ayhan, Connor Douglas, Bradley C. Lega, Genevieve Konopka

Research output: Contribution to journalArticlepeer-review

Abstract

Single-nucleus RNA sequencing (snRNA-seq), where nuclear transcriptomes are a proxy to cellular transcriptomes, has been used to profile human brain. snRNA-seq is sensitive to tissue processing, tissue quality, postmortem interval time, and cellular debris. This protocol outlines steps for the isolation of high-quality nuclei from surgically resected human brain tissue followed by a sucrose gradient yielding neuronal and non-neuronal nuclei enabling unbiased analysis of various cell types. For complete details on the use and execution of this protocol, please refer to Ayhan et al. (2021).

Original languageEnglish (US)
Article number100844
JournalSTAR Protocols
Volume2
Issue number4
DOIs
StatePublished - Dec 17 2021

Keywords

  • Cell isolation
  • Cell separation/fractionation
  • Genomics
  • Microbiology
  • Neuroscience
  • RNAseq
  • Single Cell

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)

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