Nucleophosmin/B23 is a target of CDK2/cyclin E in centrosome duplication

Masaru Okuda, Henning F. Horn, Pheruza Tarapore, Yukari Tokuyama, A. George Smulian, Pui Kwong Chan, Erik S. Knudsen, Irene A. Hofmann, Jean D. Snyder, Kevin E. Bove, Kenji Fukasawa

Research output: Contribution to journalArticlepeer-review

538 Scopus citations

Abstract

In animal cells, duplication of centrosomes and DNA is coordinated. Since CDK2/cyclin E triggers initiation of both events, activation of CDK2/cyclin E is thought to link these two events. We identified nucleophosmin (NPM/B23) as a substrate of CDK2/cyclin E in centrosome duplication. NPM/B23 associates specifically with unduplicated centrosomes, and NPM/B23 dissociates from centrosomes by CDK2/cyclin E-mediated phosphorylation. An anti-NPM/B23 antibody, which blocks this phosphorylation, suppresses the initiation of centrosome duplication in vivo. Moreover, expression of a nonphosphorylatable mutant NPM/ B23 in cells effectively blocks centrosome duplication. Thus, NPM/B23 is a target of CDK2/cyclin E in the initiation of centrosome duplication.

Original languageEnglish (US)
Pages (from-to)127-140
Number of pages14
JournalCell
Volume103
Issue number1
DOIs
StatePublished - Sep 29 2000

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Nucleophosmin/B23 is a target of CDK2/cyclin E in centrosome duplication'. Together they form a unique fingerprint.

Cite this