Nucleophosmin/B23 negatively regulates GCN5-dependent histone acetylation and transactivation

Yonglong Zou, Jun Wu, Richard J. Giannone, Lorrie Boucher, Hansen Du, Ying Huang, Dabney K. Johnson, Yie Liu, Yisong Wang

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Nucleophosmin/B23 is a multifunctional phosphoprotein that is overexpressed in cancer cells and has been shown to be involved in both positive and negative regulation of transcription. In this study, we first identified GCN5 acetyltransferase as a B23-interacting protein by mass spectrometry, which was then confirmed by in vivo co-immunoprecipitation. An in vitro assay demonstrated that B23 bound the PCAF-N domain of GCN5 and inhibited GCN5-mediated acetylation of both free and mononucleosomal histones, probably through interfering with GCN5 and masking histones from being acetylated. Mitotic B23 exhibited higher inhibitory activity on GCN5-mediated histone acetylation than interphase B23. Immunodepletion experiments of mitotic extracts revealed that phosphorylation of B23 at Thr199 enhanced the inhibition of GCN5-mediated histone acetylation. Moreover, luciferase reporter and microarray analyses suggested that B23 attenuated GCN5-mediated transactivation in vivo. Taken together, our studies suggest a molecular mechanism of B23 in the mitotic inhibition of GCN5-mediated histone acetylation and transactivation.

Original languageEnglish (US)
Pages (from-to)5728-5737
Number of pages10
JournalJournal of Biological Chemistry
Volume283
Issue number9
DOIs
StatePublished - Feb 29 2008
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Nucleophosmin/B23 negatively regulates GCN5-dependent histone acetylation and transactivation'. Together they form a unique fingerprint.

  • Cite this

    Zou, Y., Wu, J., Giannone, R. J., Boucher, L., Du, H., Huang, Y., Johnson, D. K., Liu, Y., & Wang, Y. (2008). Nucleophosmin/B23 negatively regulates GCN5-dependent histone acetylation and transactivation. Journal of Biological Chemistry, 283(9), 5728-5737. https://doi.org/10.1074/jbc.M709932200