Nucleoporin Levels Regulate Cell Cycle Progression and Phase-Specific Gene Expression

Papia Chakraborty, Yaming Wang, Jen Hsuan Wei, Jan van Deursen, Hongtao Yu, Liviu Malureanu, Mary Dasso, Douglass J. Forbes, David E. Levy, Joachim Seemann, Beatriz M A Fontoura

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

The Nup107-160 complex, the largest subunit of the nuclear pore, is multifunctional. It mediates mRNA export in interphase, and has roles in kinetochore function, spindle assembly, and postmitotic nuclear pore assembly. We report here that the levels of constituents of the Nup107-160 complex are coordinately cell cycle-regulated. At mitosis, however, a member of the complex, Nup96, is preferentially downregulated. This occurs via the ubiquitin-proteasome pathway. When the levels of Nup96 are kept high, a significant delay in G1/S progression occurs. Conversely, in cells of Nup96+/- mice, which express low levels of Nup96, cell cycle progression is accelerated. These lowered levels of Nup96 yield specific defects in nuclear export of certain mRNAs and protein expression, among which are key cell cycle regulators. Thus, Nup96 levels regulate differential gene expression in a phase-specific manner, setting the stage for proper cell cycle progression.

Original languageEnglish (US)
Pages (from-to)657-667
Number of pages11
JournalDevelopmental Cell
Volume15
Issue number5
DOIs
StatePublished - Nov 11 2008

Fingerprint

Nuclear Pore Complex Proteins
Gene expression
Cell Cycle
Cells
Gene Expression
Nuclear Pore
Kinetochores
Messenger RNA
Cell Nucleus Active Transport
Interphase
Proteasome Endopeptidase Complex
Ubiquitin
Mitosis
nuclear pore complex protein 96
Down-Regulation
Defects

Keywords

  • CELLBIO
  • CELLCYCLE

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Nucleoporin Levels Regulate Cell Cycle Progression and Phase-Specific Gene Expression. / Chakraborty, Papia; Wang, Yaming; Wei, Jen Hsuan; van Deursen, Jan; Yu, Hongtao; Malureanu, Liviu; Dasso, Mary; Forbes, Douglass J.; Levy, David E.; Seemann, Joachim; Fontoura, Beatriz M A.

In: Developmental Cell, Vol. 15, No. 5, 11.11.2008, p. 657-667.

Research output: Contribution to journalArticle

Chakraborty, P, Wang, Y, Wei, JH, van Deursen, J, Yu, H, Malureanu, L, Dasso, M, Forbes, DJ, Levy, DE, Seemann, J & Fontoura, BMA 2008, 'Nucleoporin Levels Regulate Cell Cycle Progression and Phase-Specific Gene Expression', Developmental Cell, vol. 15, no. 5, pp. 657-667. https://doi.org/10.1016/j.devcel.2008.08.020
Chakraborty, Papia ; Wang, Yaming ; Wei, Jen Hsuan ; van Deursen, Jan ; Yu, Hongtao ; Malureanu, Liviu ; Dasso, Mary ; Forbes, Douglass J. ; Levy, David E. ; Seemann, Joachim ; Fontoura, Beatriz M A. / Nucleoporin Levels Regulate Cell Cycle Progression and Phase-Specific Gene Expression. In: Developmental Cell. 2008 ; Vol. 15, No. 5. pp. 657-667.
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