TY - JOUR
T1 - Nuk controls pathfinding of commissural axons in the mammalian central nervous system
AU - Henkemeyer, Mark
AU - Orioli, Donata
AU - Henderson, Jeffrey T.
AU - Saxton, Tracy M.
AU - Roder, John
AU - Pawson, Tony
AU - Klein, Rüdiger
N1 - Funding Information:
Correspondence should be addressed to T. P. We thank D. Rossi for assistance with ES cell cultures, K. Harpel for paraffin sections, T. Calzonetti for the pATGlacZ plasmid, and G. Yancopoulos and T. Hunter for discussion. Postdoctoral support for M. H. came from the Medical Research Council of Canada (MRC) and for J. T. H. from the Rick Hansen Society. Predoctoral support for T. M. S. came from an MRC studentship and for D. O. from an EMBL fellowship. This work was supported by a grant from Bristol-Myers-Squibb, a Terry Fox programme grant from the National Cancer Institute of Canada (NCIC), a Howard Hughes International Research Scholar Award to T. P., and a grant from the Deutsche Forschungsgemeinschaft to R. K. T. P. is a Terry Fox Cancer Research Scientist of the NCIC.
PY - 1996/7/12
Y1 - 1996/7/12
N2 - Eph family receptor tyrosine kinases have been proposed to control axon guidance and fasciculation. To address the biological functions of the Eph family member Nuk, two mutations in the mouse germline have been generated: a protein null allele (Nuk1) and an allele that encodes a Nuk-βgal fusion receptor lacking the tyrosine kinase and C-terminal domains (Nuk(lacZ)). In Nuk1 homozygous brains, the majority of axons forming the posterior tract of the anterior commissure migrate aberrantly to the floor of the brain, resulting in a failure of cortical neurons to link the two temporal lobes. These results indicate that Nuk, a receptor that binds transmembrane ligands, plays a critical and unique role in the pathfinding of specific axons in the mammalian central nervous system.
AB - Eph family receptor tyrosine kinases have been proposed to control axon guidance and fasciculation. To address the biological functions of the Eph family member Nuk, two mutations in the mouse germline have been generated: a protein null allele (Nuk1) and an allele that encodes a Nuk-βgal fusion receptor lacking the tyrosine kinase and C-terminal domains (Nuk(lacZ)). In Nuk1 homozygous brains, the majority of axons forming the posterior tract of the anterior commissure migrate aberrantly to the floor of the brain, resulting in a failure of cortical neurons to link the two temporal lobes. These results indicate that Nuk, a receptor that binds transmembrane ligands, plays a critical and unique role in the pathfinding of specific axons in the mammalian central nervous system.
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U2 - 10.1016/S0092-8674(00)80075-6
DO - 10.1016/S0092-8674(00)80075-6
M3 - Article
C2 - 8689685
AN - SCOPUS:0344816258
SN - 0092-8674
VL - 86
SP - 35
EP - 46
JO - Cell
JF - Cell
IS - 1
ER -