OAS1 polymorphisms are associated with susceptibility to West Nile encephalitis in horses

Jonathan J. Rios, JoAnn G W Fleming, Uneeda K. Bryant, Craig N. Carter, John C. Huber, Maureen T. Long, Thomas E. Spencer, David L. Adelson

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

West Nile virus, first identified within the United States in 1999, has since spread across the continental states and infected birds, humans and domestic animals, resulting in numerous deaths. Previous studies in mice identified the Oas1b gene, a member of the OAS/RNASEL innate immune system, as a determining factor for resistance to West Nile virus (WNV) infection. A recent case-control association study described mutations of human OAS1 associated with clinical susceptibility to WNV infection. Similar studies in horses, a particularly susceptible species, have been lacking, in part, because of the difficulty in collecting populations sufficiently homogenous in their infection and disease states. The equine OAS gene cluster most closely resembles the human cluster, with single copies of OAS1, OAS3 and OAS2 in the same orientation. With naturally occurring susceptible and resistant sub-populations to lethal West Nile encephalitis, we undertook a case-control association study to investigate whether, similar to humans (OAS1) and mice (Oas1b), equine OAS1 plays a role in resistance to severe WNV infection. We identified naturally occurring single nucleotide mutations in equine (Equus caballus) OAS1 and RNASEL genes and, using Fisher's Exact test, we provide evidence that mutations in equine OAS1 contribute to host susceptibility. Virtually all of the associated OAS1 polymorphisms were located within the interferon-inducible promoter, suggesting that differences in OAS1 gene expression may determine the host's ability to resist clinical manifestations associated with WNV infection.

Original languageEnglish (US)
Article numbere10537
JournalPLoS One
Volume5
Issue number5
DOIs
StatePublished - 2010

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Encephalitis
West Nile virus
encephalitis
Polymorphism
Viruses
Horses
genetic polymorphism
Virus Diseases
horses
Genes
infection
mutation
Mutation
Case-Control Studies
R Factors
Immune system
Birds
Gene expression
Interferons
Domestic Animals

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Rios, J. J., Fleming, J. G. W., Bryant, U. K., Carter, C. N., Huber, J. C., Long, M. T., ... Adelson, D. L. (2010). OAS1 polymorphisms are associated with susceptibility to West Nile encephalitis in horses. PLoS One, 5(5), [e10537]. https://doi.org/10.1371/journal.pone.0010537

OAS1 polymorphisms are associated with susceptibility to West Nile encephalitis in horses. / Rios, Jonathan J.; Fleming, JoAnn G W; Bryant, Uneeda K.; Carter, Craig N.; Huber, John C.; Long, Maureen T.; Spencer, Thomas E.; Adelson, David L.

In: PLoS One, Vol. 5, No. 5, e10537, 2010.

Research output: Contribution to journalArticle

Rios, JJ, Fleming, JGW, Bryant, UK, Carter, CN, Huber, JC, Long, MT, Spencer, TE & Adelson, DL 2010, 'OAS1 polymorphisms are associated with susceptibility to West Nile encephalitis in horses', PLoS One, vol. 5, no. 5, e10537. https://doi.org/10.1371/journal.pone.0010537
Rios, Jonathan J. ; Fleming, JoAnn G W ; Bryant, Uneeda K. ; Carter, Craig N. ; Huber, John C. ; Long, Maureen T. ; Spencer, Thomas E. ; Adelson, David L. / OAS1 polymorphisms are associated with susceptibility to West Nile encephalitis in horses. In: PLoS One. 2010 ; Vol. 5, No. 5.
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