Occult hepatitis B viral DNA in liver carcinomas from a region with a low prevalence of chronic hepatitis B infection

R. Kannangai, E. Molmenti, L. Arrazola, A. Klein, M. Choti, D. L. Thomas, M. Torbenson, Michael Torbenson

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Occult hepatitis B is defined by the presence of hepatitis B viral (HBV) DNA in the serum or liver in persons lacking hepatitis B surface antigen (HBsAg) in the serum. A high prevalence of occult HBV has been reported in hepatocellular carcinoma (HCC) from Asia, but little information is available on the prevalence of occult HBV in HCC from regions with a low prevalence of typical chronic hepatitis B infection. In a retrospective study, 19 cases of primary liver cancer were investigated for the presence of occult HBV DNA by amplification of the surface, core, and X gene. In addition, HBV copy numbers were quantitated by real time polymerase chain reaction, genotyped, and samples tested for covalently closed circular HBV DNA, which is a marker of active viral replication. Occult HBV was found in three of 19 cases (16%). Genotyping was successful in two cases, both of which were genotype A. HBV DNA copy numbers were low, all less than 10 copies/μg liver DNA. No closed circular HBV DNA was detected. Thus, in this study occult HBV was of genotype A and was found in a low percentage of cases of HCC and was associated with low tissue HBV DNA copy numbers and no detectable evidence for viral replication.

Original languageEnglish (US)
Pages (from-to)297-301
Number of pages5
JournalJournal of Viral Hepatitis
Volume11
Issue number4
DOIs
StatePublished - Jul 2004

Fingerprint

Viral DNA
Chronic Hepatitis B
Hepatitis B
Carcinoma
Liver
Infection
Hepatocellular Carcinoma
Genotype
Liver Neoplasms
Hepatitis B Surface Antigens
Serum
Real-Time Polymerase Chain Reaction
Retrospective Studies
Biomarkers

Keywords

  • Cholangiocarcinoma
  • Hepatocellular carcinoma
  • Occult hepatitis B

ASJC Scopus subject areas

  • Hepatology
  • Virology

Cite this

Occult hepatitis B viral DNA in liver carcinomas from a region with a low prevalence of chronic hepatitis B infection. / Kannangai, R.; Molmenti, E.; Arrazola, L.; Klein, A.; Choti, M.; Thomas, D. L.; Torbenson, M.; Torbenson, Michael.

In: Journal of Viral Hepatitis, Vol. 11, No. 4, 07.2004, p. 297-301.

Research output: Contribution to journalArticle

Kannangai, R, Molmenti, E, Arrazola, L, Klein, A, Choti, M, Thomas, DL, Torbenson, M & Torbenson, M 2004, 'Occult hepatitis B viral DNA in liver carcinomas from a region with a low prevalence of chronic hepatitis B infection', Journal of Viral Hepatitis, vol. 11, no. 4, pp. 297-301. https://doi.org/10.1111/j.1365-2893.2004.00502.x
Kannangai, R. ; Molmenti, E. ; Arrazola, L. ; Klein, A. ; Choti, M. ; Thomas, D. L. ; Torbenson, M. ; Torbenson, Michael. / Occult hepatitis B viral DNA in liver carcinomas from a region with a low prevalence of chronic hepatitis B infection. In: Journal of Viral Hepatitis. 2004 ; Vol. 11, No. 4. pp. 297-301.
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