OLIG2 over-expression impairs proliferation of human down syndrome neural progenitors

Jie Lu, Gewei Lian, Hui Zhou, Giuseppe Esposito, Luca Steardo, Laurent C. Delli-Bovi, Jonathan L. Hecht, Q. Richard Lu, Volney Sheen

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Mental retardation and early Alzheimer's disease (AD) have generally been attributed to progressive neuronal loss in the developing and mature Down syndrome (DS) brain. However, reduced neuronal production during development could also contribute to the smaller brain size and simplified gyral patterning seen in this disorder. Here, we show impairments in proliferation within the ventricular zone (VZ) of early DS fetal cortex and in cultured early passage DS human neural progenitors (HNPs). We find that the reduced proliferative rates correspond temporally with increased expression of the chromosome 21 (HSA21) associated, oligodendrocyte transcription factor OLIG2 at 14-18 weeks gestational age (GA) (period of neurogenesis). Moreover, the DS HNPs adopt more oligodendrocyte-specific features including increased oligodendrocyte marker expression, as well as a reduction in KCNA3 potassium channel expression and function. We further show that OLIG2 inhibition or over-expression regulates potassium channel expression levels and that activation or inhibition of these channels influences the rate of progenitor proliferation. Finally, neural progenitors from Olig2 over-expressing transgenic mice exhibit these same impairments in proliferation and potassium channel expression. These findings suggest that OLIG2 over-expression inhibits neural progenitor proliferation through changes in potassium channel activity, thereby contributing to the reduced neuronal numbers and brain size in DS.

Original languageEnglish (US)
Article numberdds052
Pages (from-to)2330-2340
Number of pages11
JournalHuman Molecular Genetics
Volume21
Issue number10
DOIs
StatePublished - May 2012

Fingerprint

Down Syndrome
Potassium Channels
Oligodendroglia
Kv1.3 Potassium Channel
Brain
Chromosomes, Human, Pair 21
Neurogenesis
Intellectual Disability
Transgenic Mice
Gestational Age
Alzheimer Disease
Transcription Factors
Inhibition (Psychology)

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Molecular Biology

Cite this

Lu, J., Lian, G., Zhou, H., Esposito, G., Steardo, L., Delli-Bovi, L. C., ... Sheen, V. (2012). OLIG2 over-expression impairs proliferation of human down syndrome neural progenitors. Human Molecular Genetics, 21(10), 2330-2340. [dds052]. https://doi.org/10.1093/hmg/dds052

OLIG2 over-expression impairs proliferation of human down syndrome neural progenitors. / Lu, Jie; Lian, Gewei; Zhou, Hui; Esposito, Giuseppe; Steardo, Luca; Delli-Bovi, Laurent C.; Hecht, Jonathan L.; Lu, Q. Richard; Sheen, Volney.

In: Human Molecular Genetics, Vol. 21, No. 10, dds052, 05.2012, p. 2330-2340.

Research output: Contribution to journalArticle

Lu, J, Lian, G, Zhou, H, Esposito, G, Steardo, L, Delli-Bovi, LC, Hecht, JL, Lu, QR & Sheen, V 2012, 'OLIG2 over-expression impairs proliferation of human down syndrome neural progenitors', Human Molecular Genetics, vol. 21, no. 10, dds052, pp. 2330-2340. https://doi.org/10.1093/hmg/dds052
Lu J, Lian G, Zhou H, Esposito G, Steardo L, Delli-Bovi LC et al. OLIG2 over-expression impairs proliferation of human down syndrome neural progenitors. Human Molecular Genetics. 2012 May;21(10):2330-2340. dds052. https://doi.org/10.1093/hmg/dds052
Lu, Jie ; Lian, Gewei ; Zhou, Hui ; Esposito, Giuseppe ; Steardo, Luca ; Delli-Bovi, Laurent C. ; Hecht, Jonathan L. ; Lu, Q. Richard ; Sheen, Volney. / OLIG2 over-expression impairs proliferation of human down syndrome neural progenitors. In: Human Molecular Genetics. 2012 ; Vol. 21, No. 10. pp. 2330-2340.
@article{d2f7b1856b12479b89d63970c91b06dc,
title = "OLIG2 over-expression impairs proliferation of human down syndrome neural progenitors",
abstract = "Mental retardation and early Alzheimer's disease (AD) have generally been attributed to progressive neuronal loss in the developing and mature Down syndrome (DS) brain. However, reduced neuronal production during development could also contribute to the smaller brain size and simplified gyral patterning seen in this disorder. Here, we show impairments in proliferation within the ventricular zone (VZ) of early DS fetal cortex and in cultured early passage DS human neural progenitors (HNPs). We find that the reduced proliferative rates correspond temporally with increased expression of the chromosome 21 (HSA21) associated, oligodendrocyte transcription factor OLIG2 at 14-18 weeks gestational age (GA) (period of neurogenesis). Moreover, the DS HNPs adopt more oligodendrocyte-specific features including increased oligodendrocyte marker expression, as well as a reduction in KCNA3 potassium channel expression and function. We further show that OLIG2 inhibition or over-expression regulates potassium channel expression levels and that activation or inhibition of these channels influences the rate of progenitor proliferation. Finally, neural progenitors from Olig2 over-expressing transgenic mice exhibit these same impairments in proliferation and potassium channel expression. These findings suggest that OLIG2 over-expression inhibits neural progenitor proliferation through changes in potassium channel activity, thereby contributing to the reduced neuronal numbers and brain size in DS.",
author = "Jie Lu and Gewei Lian and Hui Zhou and Giuseppe Esposito and Luca Steardo and Delli-Bovi, {Laurent C.} and Hecht, {Jonathan L.} and Lu, {Q. Richard} and Volney Sheen",
year = "2012",
month = "5",
doi = "10.1093/hmg/dds052",
language = "English (US)",
volume = "21",
pages = "2330--2340",
journal = "Human Molecular Genetics",
issn = "0964-6906",
publisher = "Oxford University Press",
number = "10",

}

TY - JOUR

T1 - OLIG2 over-expression impairs proliferation of human down syndrome neural progenitors

AU - Lu, Jie

AU - Lian, Gewei

AU - Zhou, Hui

AU - Esposito, Giuseppe

AU - Steardo, Luca

AU - Delli-Bovi, Laurent C.

AU - Hecht, Jonathan L.

AU - Lu, Q. Richard

AU - Sheen, Volney

PY - 2012/5

Y1 - 2012/5

N2 - Mental retardation and early Alzheimer's disease (AD) have generally been attributed to progressive neuronal loss in the developing and mature Down syndrome (DS) brain. However, reduced neuronal production during development could also contribute to the smaller brain size and simplified gyral patterning seen in this disorder. Here, we show impairments in proliferation within the ventricular zone (VZ) of early DS fetal cortex and in cultured early passage DS human neural progenitors (HNPs). We find that the reduced proliferative rates correspond temporally with increased expression of the chromosome 21 (HSA21) associated, oligodendrocyte transcription factor OLIG2 at 14-18 weeks gestational age (GA) (period of neurogenesis). Moreover, the DS HNPs adopt more oligodendrocyte-specific features including increased oligodendrocyte marker expression, as well as a reduction in KCNA3 potassium channel expression and function. We further show that OLIG2 inhibition or over-expression regulates potassium channel expression levels and that activation or inhibition of these channels influences the rate of progenitor proliferation. Finally, neural progenitors from Olig2 over-expressing transgenic mice exhibit these same impairments in proliferation and potassium channel expression. These findings suggest that OLIG2 over-expression inhibits neural progenitor proliferation through changes in potassium channel activity, thereby contributing to the reduced neuronal numbers and brain size in DS.

AB - Mental retardation and early Alzheimer's disease (AD) have generally been attributed to progressive neuronal loss in the developing and mature Down syndrome (DS) brain. However, reduced neuronal production during development could also contribute to the smaller brain size and simplified gyral patterning seen in this disorder. Here, we show impairments in proliferation within the ventricular zone (VZ) of early DS fetal cortex and in cultured early passage DS human neural progenitors (HNPs). We find that the reduced proliferative rates correspond temporally with increased expression of the chromosome 21 (HSA21) associated, oligodendrocyte transcription factor OLIG2 at 14-18 weeks gestational age (GA) (period of neurogenesis). Moreover, the DS HNPs adopt more oligodendrocyte-specific features including increased oligodendrocyte marker expression, as well as a reduction in KCNA3 potassium channel expression and function. We further show that OLIG2 inhibition or over-expression regulates potassium channel expression levels and that activation or inhibition of these channels influences the rate of progenitor proliferation. Finally, neural progenitors from Olig2 over-expressing transgenic mice exhibit these same impairments in proliferation and potassium channel expression. These findings suggest that OLIG2 over-expression inhibits neural progenitor proliferation through changes in potassium channel activity, thereby contributing to the reduced neuronal numbers and brain size in DS.

UR - http://www.scopus.com/inward/record.url?scp=84860468803&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860468803&partnerID=8YFLogxK

U2 - 10.1093/hmg/dds052

DO - 10.1093/hmg/dds052

M3 - Article

VL - 21

SP - 2330

EP - 2340

JO - Human Molecular Genetics

JF - Human Molecular Genetics

SN - 0964-6906

IS - 10

M1 - dds052

ER -