Olig2 targets chromatin remodelers to enhancers to initiate oligodendrocyte differentiation

Yang Yu, Ying Chen, Bongwoo Kim, Haibo Wang, Chuntao Zhao, Xuelian He, Lei Liu, Wei Liu, Lai Man N Wu, Meng Mao, Jonah R. Chan, Jiang Wu, Q. Richard Lu

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

Establishment of oligodendrocyte identity is crucial for subsequent events of myelination in the CNS. Here, we demonstrate that activation of ATP-dependent SWI/SNF chromatin-remodeling enzyme Smarca4/Brg1 at the differentiation onset is necessary and sufficient to initiate and promote oligodendrocyte lineage progression and maturation. Genome-wide multistage studies by ChIP-seq reveal that oligodendrocyte-lineage determination factor Olig2 functions as a prepatterning factor to direct Smarca4/Brg1 to oligodendrocyte-specific enhancers. Recruitment of Smarca4/Brg1 to distinct subsets of myelination regulatory genes is developmentally regulated. Functional analyses of Smarca4/Brg1 and Olig2 co-occupancy relative to chromatin epigenetic marking uncover stage-specific cis-regulatory elements that predict sets of transcriptional regulators controlling oligodendrocyte differentiation. Together, our results demonstrate that regulation of the functional specificity and activity of a Smarca4/Brg1-dependent chromatin-remodeling complex by Olig2, coupled with transcriptionally linked chromatin modifications, is critical to precisely initiate and establish the transcriptional program that promotes oligodendrocyte differentiation and subsequent myelination of the CNS.

Original languageEnglish (US)
Pages (from-to)248-261
Number of pages14
JournalCell
Volume152
Issue number1-2
DOIs
StatePublished - Jan 7 2013

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Oligodendroglia
Chromatin
Chromatin Assembly and Disassembly
Genes
Adenosine Triphosphate
Chemical activation
Regulator Genes
Epigenomics
Enzymes
Genome

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Yu, Y., Chen, Y., Kim, B., Wang, H., Zhao, C., He, X., ... Lu, Q. R. (2013). Olig2 targets chromatin remodelers to enhancers to initiate oligodendrocyte differentiation. Cell, 152(1-2), 248-261. https://doi.org/10.1016/j.cell.2012.12.006

Olig2 targets chromatin remodelers to enhancers to initiate oligodendrocyte differentiation. / Yu, Yang; Chen, Ying; Kim, Bongwoo; Wang, Haibo; Zhao, Chuntao; He, Xuelian; Liu, Lei; Liu, Wei; Wu, Lai Man N; Mao, Meng; Chan, Jonah R.; Wu, Jiang; Lu, Q. Richard.

In: Cell, Vol. 152, No. 1-2, 07.01.2013, p. 248-261.

Research output: Contribution to journalArticle

Yu, Y, Chen, Y, Kim, B, Wang, H, Zhao, C, He, X, Liu, L, Liu, W, Wu, LMN, Mao, M, Chan, JR, Wu, J & Lu, QR 2013, 'Olig2 targets chromatin remodelers to enhancers to initiate oligodendrocyte differentiation', Cell, vol. 152, no. 1-2, pp. 248-261. https://doi.org/10.1016/j.cell.2012.12.006
Yu, Yang ; Chen, Ying ; Kim, Bongwoo ; Wang, Haibo ; Zhao, Chuntao ; He, Xuelian ; Liu, Lei ; Liu, Wei ; Wu, Lai Man N ; Mao, Meng ; Chan, Jonah R. ; Wu, Jiang ; Lu, Q. Richard. / Olig2 targets chromatin remodelers to enhancers to initiate oligodendrocyte differentiation. In: Cell. 2013 ; Vol. 152, No. 1-2. pp. 248-261.
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